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目的研究氯化镧对小鼠大脑皮质星形胶质细胞凋亡相关基因表达的影响。方法原代培养的小鼠大脑皮质星形胶质细胞分别于终浓度为0(对照)、0.25、0.5、1.0 mmol/L氯化镧无血清培养基处理24 h后,采用RT-PCR法检测星形胶质细胞B细胞淋巴瘤2(B-cell lymphoma 2,Bcl-2)、B细胞淋巴瘤2相关X基因(Bcl-2-associated Xgene,Bax)、半胱氨酸天冬氨酸蛋白酶-3(caspase-3)、caspase-8和caspase-9 mRNA的表达水平。结果与对照组比较,0.25、0.5和1.0 mmol/L氯化镧染毒小鼠星形胶质细胞Bax、caspase-3和caspase-9 mRNA的表达水平均显著升高,0.5和1.0 mmol/L氯化镧染毒小鼠星形胶质细胞Bcl-2 mRNA的表达水平均显著降低,差异有统计学意义(P<0.05);且随着氯化镧染毒剂量的升高,小鼠星形胶质细胞Bax、caspase-3和caspase-9 mRNA的表达水平均呈上升趋势,Bcl-2 mRNA的表达水平呈下降趋势。而各组小鼠星形胶质细胞caspase-8 mRNA的表达水平间比较,差异无统计学意义。结论氯化镧对星形胶质细胞产生毒作用的机制可能与氯化镧致促凋亡基因Bax和caspase-3、caspase-9表达升高以及抑凋亡基因Bcl-2表达下降有关。
Objective To study the effects of lanthanum chloride on the expression of apoptosis related genes in cerebral cortex astrocytes in mice. Methods Primary cultures of mouse cerebral cortical astrocytes were treated with serum-free medium at concentrations of 0 (control), 0.25, 0.5 and 1.0 mmol / L lanthanum chloride for 24 h, respectively. RT-PCR was used to detect B-cell lymphoma 2 (Bcl-2), Bcl-2-associated Xgene (Bax), cysteine aspartic proteinase -3 (caspase-3), caspase-8 and caspase-9 mRNA expression levels. Results Compared with the control group, the expression levels of Bax, caspase-3 and caspase-9 mRNA in astrocytes induced by lanthanum chloride at 0.25, 0.5 and 1.0 mmol / L were significantly increased at 0.5, 1.0 mmol / L The expression level of Bcl-2 mRNA in astrocytes induced by lanthanum chloride was significantly decreased (P <0.05), and with the dose of lanthanum chloride increased, the expression of mouse star The expression of Bax, caspase-3 and caspase-9 mRNAs in astrocytes increased and the expression of Bcl-2 mRNA decreased. There was no significant difference in the expression levels of caspase-8 mRNA between astrocytes in each group. Conclusion The mechanism of toxic effects of lanthanum chloride on astrocytes may be related to the increase of the expressions of Bax, caspase-3 and caspase-9 and the decrease of Bcl-2 expression induced by lanthanum chloride.