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目的:研究SKP2、P27、Galectin-3在子宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)中的表达及意义。方法:采用免疫组化二步法检测SKP2、P27、Galectin-3蛋白在CIN60例、慢性宫颈炎20例、正常宫颈20例中的表达。结果:SKP2、Galectin-3阳性细胞主要分布在上皮内瘤变的鳞状细胞及部分基底细胞中,而P27阳性细胞主要分布在中表层非上皮内瘤变的鳞状细胞中。SKP2、Galectin-3在CINⅠ、Ⅱ、Ⅲ组中的阳性率明显高于正常宫颈、慢性宫颈炎组(P<0.05),其阳性率随CIN级别的增加而升高。SKP2在CINⅢ组中的阳性率明显高于CINⅠ组(P<0.05)。P27在CINⅡ、Ⅲ组中的阳性率明显低于正常宫颈、慢性宫颈炎组(P<0.05)。SKP2、Galectin-3两者与P27阳性表达呈负相关,其阳性表达在CIN中与患者民族、年龄无关(P>0.05)。结论:随宫颈CIN级别的增加,SKP2高表达而P27低表达,两者呈负相关,与宫颈恶性肿瘤的发生、发展有关。SKP2、P27联合检测有助于慢性宫颈炎与CIN的鉴别。Galectin-3在宫颈CIN中可能起促进作用。
Objective: To study the expression and significance of SKP2, P27 and Galectin-3 in cervical intraepithelial neoplasia (CIN). Methods: The expressions of SKP2, P27 and Galectin-3 protein in 60 cases of CIN, 20 cases of chronic cervicitis and 20 cases of normal cervix were detected by immunohistochemical two-step method. Results: The positive cells of SKP2 and Galectin-3 mainly distributed in squamous cells and some basal cells of intraepithelial neoplasia, whereas the positive cells of P27 mainly distributed in the non-intraepithelial neoplasia of squamous cell. The positive rates of SKP2 and Galectin-3 in CINⅠ, Ⅱ and Ⅲgroups were significantly higher than those in normal cervix and chronic cervicitis group (P <0.05), and the positive rates of SKP2 and Galectin-3 increased with the increase of CIN level. The positive rate of SKP2 in CINⅢgroup was significantly higher than that in CINⅠgroup (P <0.05). The positive rate of P27 in CINⅡ and Ⅲgroups was significantly lower than that in normal cervix and chronic cervicitis group (P <0.05). The positive expression of SKP2 and Galectin-3 was negatively correlated with the positive expression of P27. The positive expression of SKP2 and Galectin-3 in CIN was not related to the ethnicity and age (P> 0.05). Conclusion: With the increase of cervical CIN level, SKP2 is overexpressed and P27 is low, which is negatively correlated with the occurrence and development of cervical malignant tumors. SKP2, P27 joint detection contribute to the identification of chronic cervicitis and CIN. Galectin-3 may play a catalytic role in cervical CIN.