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目的:建立测定银黄清火胶囊中绿原酸含量的方法。方法:采用高效液相色谱法。色谱柱为Acclaim120?C18,流动相为乙腈-0.4%磷酸(12∶88,V/V),流速为1 ml/min,检测波长为327 nm,柱温为25℃,进样量为10μl。结果:绿原酸检测进样量线性范围为56~224μg;精密度、稳定性、重复性的RSD<3.0%;加样回收率为96.91%~104.76%,RSD为1.46%(n=9)。结论:该方法操作简便、重复性好,可用于银黄清火胶囊的质量控制。
Objective: To establish a method for determination of chlorogenic acid in Yinhuang Qinghuo capsule. Methods: Using high performance liquid chromatography. The chromatographic column was Acclaim 120 C18 with a mobile phase of acetonitrile-0.4% phosphoric acid (12:88 V / V) at a flow rate of 1 ml / min with a detection wavelength of 327 nm and a column temperature of 25 ° C. Results: The linear range of chlorogenic acid was 56 ~ 224μg, the RSD of precision, stability and repeatability was less than 3.0%. The recoveries were 96.91% ~ 104.76% with RSD of 1.46% (n = 9) . Conclusion: The method is simple, reproducible and can be used for the quality control of Yinhuang Qinghuo capsule.