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由致病疫霉菌引起的马铃薯晚疫病是马铃薯生长期和储藏期的一种毁灭性病害。PCR检测技术已成为检测晚疫病致病菌的一种常规方法。本研究利用9对国内外已发表的晚疫病检测引物,通过对致病疫霉菌和其他5种常见的侵染马铃薯的病原真菌进行PCR检测,筛选出Primer-4-2和Primer-5对晚疫病菌的特异性较好,而其他7对引物特异性较差或不具有特异性。在对带病薯块、植株及组培苗的样品检测中,引物Primer-4-2特异性优于Primer-5,能够实现对不同晚疫病样品的检测,其引物灵敏度检测为10 pg/μL。
Late blight caused by Phytophthora infestans is a devastating disease in potato growth and storage. PCR detection technology has become a routine method of detecting late blight pathogens. In this study, nine pairs of primers for late blight disease were reported in China. Primer-4-2 and Primer-5 were screened by polymerase chain reaction (PCR) against Phytophthora infestans and five other pathogenic fungi that infest potato. Phytophthora is more specific, while the other seven pairs are less specific or non-specific. The primer Primer-4-2 was superior to Primer-5 in the detection of samples with diseased tubers, plants and tissue culture seedlings, and could detect different late blight samples with the sensitivity of 10 pg / μL .