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目的 观察茶多酚（ｔｅａ ｐｏｌｙｐｈｅｎｏｌｓ， ＴＰ） 对人鼻咽癌细胞株ＣＮＥ２ 细胞ＤＮＡ 的损伤作用和细胞周期的影响。方法 用琼脂糖凝胶电泳方法测定ＴＰ对细胞ＤＮＡ的断裂作用；用流式细胞术（ＦＣＭ） 观察ＴＰ对ＣＮＥ２ 细胞周期的变化及其诱导凋亡。结果 ＴＰ可引起ＣＮＥ２ 细胞ＤＮＡ 断裂、其断裂程度随剂量的增加和作用时间的延长而增强。ＴＰ不同浓度和不同作用时间可干扰ＣＮＥ２ 细胞在周期中的进程，使Ｇ１ 期细胞明显增多，Ｓ期细胞减少，细胞分裂增殖指数（ＰＩ）亦随之降低。同时，ＴＰ可诱导ＣＮＥ２ 细胞凋亡，其凋亡率随ＴＰ浓度的增加和作用时间的延长在逐渐增加。结论 ＴＰ１９９８１２０４ 收稿，１９９９０７３１ 修回＊ 国家自然科学基金资助课题，Ｎｏ ３９３７０８００作者简介：谢冰芬，女，副研究员，硕士生导师，研究方向为肿瘤药理可引起人鼻咽癌细胞株ＣＮＥ２ 细胞的ＤＮＡ 断裂，使细胞停滞于Ｇ１ 期，阻止Ｇ１ 期细胞进入Ｓ期；同时亦可诱导细胞凋亡，这些结果可能为ＴＰ的抗瘤作用提供理论依据
Objective To observe the effect of tea polyphenols (TP) on DNA damage and cell cycle of human nasopharyngeal carcinoma cell line CNE2. Methods The DNA fragmentation of TP was detected by agarose gel electrophoresis. The change of cell cycle of CNE2 and the induction of apoptosis were observed by flow cytometry (FCM). Results TP could induce DNA fragmentation in CNE2 cells, and its degree of fragmentation increased with increasing dose and duration of action. Different concentrations of TP and different durations of action can interfere with the progression of CNE2 cells during the cycle, leading to a significant increase in G1 phase cells, a decrease in S phase cells, and a decrease in cell division and proliferation index (PI). At the same time, TP can induce apoptosis of CNE2 cells, and its apoptosis rate gradually increases with the increase of TP concentration and the prolongation of the action time. Conclusions TP19981204 Received, 19990731 Revised* National Natural Science Foundation of China, No 39370800 Author’s profile: Xie Bingfen, female, associate research fellow, master tutor, research on cancer pharmacology can cause human nasopharyngeal DNA fragmentation in cancer cell line CNE2 cells arrests cells in G1 phase, prevents G1 phase cells from entering S phase, and induces cell apoptosis. These results may provide a theoretical basis for the anti-tumor effect of TP.