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目的 :探讨小儿体外循环术中抑肽酶对IL 10的影响及量效关系。方法 :按入选标准筛选病例并分成三组 :对照组 (A组 ,n =12 )、小剂量抑肽酶组 (B组 ,n =12 )、大剂量抑肽酶组 (C组 ,n =2 0 )。用酶联免疫吸附试验法测定各患儿在术前、体外循环 (ECC) 3 0min、ECC结束后 2h、2 4h、7d五个时间点血清IL 10的浓度。结果 :各组ECC3 0min ,ECC结束后 2h血清IL 10浓度与术前相比显著增高 (P <0 .0 1) ,且B、C组与A组相比 ,C组与B组相比 ,IL 10水平增高更为明显 ,各组间差异有显著性 (P <0 .0 1) ;C组术后住院日明显缩短 (P <0 .0 1)。结论 :抑肽酶能促进内源性IL 10的释放 ,从而抑制小儿ECC引起的全身炎性反应 ,且药效与抑肽酶剂量呈正相关
Objective: To investigate the effect of aprotinin on IL-10 in pediatric cardiopulmonary bypass and its dose-response relationship. Methods: According to the inclusion criteria, the patients were divided into three groups: control group (group A, n = 12), low dose aprotinin group (group B, n = 12), high dose aprotinin group (group C, n = 2 0). The levels of IL-10 in serum were detected by enzyme-linked immunosorbent assay (ELISA) at preoperative, extracorporeal circulation (ECC) 30 min, ECC 2 h, 24 h, 7 d respectively. Results: The concentrations of serum IL-10 in ECC3 at 0min and 2h after ECC were significantly higher than those before operation (P <0.01). Compared with group A, group C and C, The level of IL-10 increased more significantly, with significant difference between groups (P <0.01). The length of stay in hospital after C group was significantly shorter (P <0.01). CONCLUSION: Aprotinin can promote the release of endogenous IL-10, thereby inhibiting systemic inflammatory response induced by ECC in children, and the pharmacodynamics is positively correlated with the dosage of aprotinin