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前言脲酶电极是目前研究得较多的酶电极之一,一般是由适当的基础电极与固定化脲酶膜组合而成,作为基础电极主要有 NH_4~+离子电极,pH 电极,CO_2电极及氨气敏电极,而脲酶电极的固定化方法则曾用过聚丙烯酰胺包埋法,PVC(聚氯乙烯)包埋法,PVA(聚乙烯醇)包埋法及戊二醛交联法等。为了使该酶电极能更好地适应临床检验及食品分析的要求,并尽快将脲酶电极推广应用,我们对6种不同载体固定化方法,3种不同骨架电极及不同测试条件对电极性能的影响作了系统研究。结果表明,当采用化学交联法将脲酶固定在尼龙网上,用氨气敏电极作骨架电极时制得的脲酶电极性能最优;当选用 pH 为8.0～8.5的磷酸盐缓冲液时,电极在
Introduction Urease electrode is currently one of the more studied electrode, usually by the appropriate base electrode and the combination of immobilized urease membrane, as the basic electrode are NH_4 ~ + ion electrode, pH electrode, CO_2 electrode and ammonia Sensitive electrode, while the urease electrode immobilization methods have been used polyacrylamide embedding method, PVC (polyvinyl chloride) embedding method, PVA (polyvinyl alcohol) embedding and glutaraldehyde crosslinking. In order to make the electrode more suitable for clinical examination and food analysis, and promote the application of urease electrode as soon as possible, we studied the effect of six different immobilization methods, three different framework electrodes and different test conditions on the performance of the electrode Made a systematic study. The results showed that when urease was immobilized on nylon net by chemical cross-linking method, urease electrode with ammonia gas sensing electrode was the best. When using phosphate buffer solution with pH 8.0-8.5,