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目的研究不同时期胚胎对胚胎移植成功率的影响,以提高胚胎移植率。方法采用经超数排卵的KM雌鼠与正常615雄鼠交配,取受精卵,分别将1-细胞胚、2-细胞胚和桑椹胚、囊胚分别移入发情的假孕KM雌鼠的输卵管和子宫中,妊娠产仔。结果移入1-细胞胚、2-细胞胚、桑椹胚和囊胚的假孕母鼠妊娠率分别为36.36%、42.86%、10%、27.27%,产仔率分别为46.58%、38.46%、58.33%、60%。结论2-细胞胚的移植可提高小鼠的胚胎移植成功率,子宫移植可提高小鼠的产仔率。
Objective To study the effect of different embryos on the success rate of embryo transfer in order to improve the rate of embryo transfer. Methods Female KM mice with superovulation were mated with 615 normal male mice and the fertilized eggs were taken. The 1-cell embryos, 2-cell embryos and morulae and blastocysts were transferred into oviducts and ovaries of estrogen- Uterus, pregnancy litter size. Results The pregnancy rates of pseudopregnant mothers transferred into 1-cell embryos, 2-cell embryos, morulae and blastocysts were 36.36%, 42.86%, 10%, 27.27%, and the litter size were 46.58%, 38.46%, 58.33 %, 60%. Conclusion Transplantation of 2-cell embryos can improve the success rate of embryo transfer in mice. Uterine transplantation can increase the birth rate of mice.