成石前后胆囊粘膜上皮多聚核蛋白体RNA含量变化—胆液糖蛋白和蛋白质增加的机制探讨

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胆囊淤泥是胆囊结石的前身。测定胆囊粘膜上皮游离(FPR)和结合多聚核蛋白体(MBPR)的RNA含量及胆液粘液糖蛋白(MGP)的结果表明:胆囊淤泥患者胆囊粘膜合成和分泌MGP明显亢进,MBPR与相应GB中MGP的相关性比较提示此时尚有胆液淤积存在,且FPR的RNA含量也显著增高,提示细胞增殖加快。荧光胺法测定胆液蛋白质的含量发现,胆囊淤泥和胆固醇性结石患者胆囊液GB蛋白质含量显著高于色素性结石患者及“正常”对照,但蛋白质的GB浓度与HB浓度的比值显著低于胆固醇GB/HB比值,且GB中MGP与蛋白质含量呈显著正相关。说明蛋白质含量的增加与GB中过高的MGP阻止胆囊中蛋白质的清除过程有关。成石前GB中MGP和蛋白质含量的同时增高及其机制的确立,对认识及预防体内结石均有意义。 Gallbladder sludge is the predecessor of gallstone. The results of determination of GFP and MGP in gallbladder mucosa epithelial free (FPR) and bound polyribosin (MBPR) showed that gallbladder mucosa synthesis and secretion of MGP were significantly increased, The correlation between MGP suggests that there is still gallstone deposition at this time, and the FPR RNA content was also significantly increased, suggesting that cell proliferation accelerated. Fluorescene amine method for the determination of protein content in gallbladder found that gallbladder sludge and gallstone patients gallbladder fluid GB protein content was significantly higher than the pigmented stone patients and the “normal” control, but the protein GB concentration and HB concentration ratio was significantly lower than the cholesterol GB / HB ratio, and there is a significant positive correlation between MGP and protein content in GB. Explain the increase in protein content and GB in the high MGP to prevent the gallbladder protein clearance process. The formation of MGP and protein content in GB before the formation of stone and the establishment of its mechanism are of great significance for understanding and preventing stones in the body.
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