论文部分内容阅读
目的比较慢性重型乙型肝炎(CSHB)与慢性乙型肝炎(CHB)患者HBV前S/S蛋白特异性细胞毒性T细胞(CTL)表位变异的差异,探讨乙型肝炎重症化和慢性化的机制。方法对262例乙型肝炎患者的血清样本进行HLA-A2分型;用巢式PCR扩增血清HBV前S/S基因并对PCR产物进行序列测定;根据HBV前S/S基因序列,用VirusBlast软件鉴定患者感染的HBV基因型;用VectorNTI软件对目前已知的13个HLA-A2限制性前S/S蛋白特异性CTL表位进行序列分析。结果123例(46.9%)患者HLA-A2阳性,其中CSHB71例,CHB52例。CTL表位变异分析结果如下:(1)两组间所有患者进行比较,患者S177-185和S338-347表位变异发生率有显著差异(P<0.05);(2)两组间HBVB基因型患者进行比较,患者S131-139、S183-191和S204-212表位变异发生率有极显著差异(P<0.01);(3)两组间HBVC基因型患者进行比较,CSHB组患者的S131-139表位较CHB组患者有增高(P=0.05)。结论某些HBV前S/S蛋白特异性CTL表位在CSHB与CHB患者间变异有明显差异,受病毒基因型影响,CTL表位变异可能与乙型肝炎的重症化和慢性化机制相关。
Objective To compare the differences of HBV preS / S specific cytotoxic T lymphocyte (CTL) epitopes between patients with chronic severe hepatitis B (CSHB) and chronic hepatitis B (CHB) mechanism. Methods The serum samples of 262 hepatitis B patients were genotyped by HLA-A2. The serum HBV pre-S / S gene was amplified by nested PCR and the PCR products were sequenced. According to the HBV pre-S / S gene sequence, Software was used to identify HBV genotypes in patients. VectorNTI software was used to analyze the 13 known HLA-A2 restricted pre-S / S protein-specific CTL epitopes. Results 123 cases (46.9%) were positive for HLA-A2, including 71 cases of CSHB and 52 cases of CHB. The results of CTL epitope variation analysis were as follows: (1) All patients in both groups had significant differences in the incidence of S177-185 and S338-347 (P <0.05); (2) HBVB genotype (P <0.01). (3) The patients with HBVC genotypes were compared between the two groups, and the S131-S131-139, S183-191 and S204-212 patients in the CSHB group were significantly different 139 epitopes were higher in CHB patients (P = 0.05). Conclusion Some HBV preS / S protein specific CTL epitopes have significant differences among patients with CSHB and CHB. Influenced by the virus genotype, CTL epitope variation may be related to the severe and chronic hepatitis B mechanism.