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本文研究了各种影响酶促反应速度的因素对从猪、牛肾中分离得到的氨基酰化酶拆分ＤＬ－Ａｌａ制备Ｄ－Ａｌａ的影响，结果表明，酶促反应的最适条件为：Ｎ－乙酰－ＤＬ－Ａｌａ的浓度为０．２ｍｏｌ／Ｌ，酶用量７００ｕ／ｍｍｏｌ底物，反应温度３７℃，ｐＨ７．０，反应时间５ｈ。牛肾氨基酰化酶和猪肾氨基酰化酶的拆分率和Ｄ－Ａｌａ得率最高分别为９０％和８４％；８９．７％和６６．１％，表明牛肾氨基酰化酶的拆分率和得率均优于猪肾氨基酰化酶。
In this paper, we studied the effects of various factors affecting the rate of enzymatic reaction on the separation of D-Ala from DL-Ala by aminoacylase isolated from porcine and bovine kidney. The results showed that the optimal conditions for enzymatic reaction were as follows: The concentration of N-acetyl-DL-Ala was 0.2mol / L, enzyme dosage 700u / mmol substrate, the reaction temperature 37 ℃, pH7.0, reaction time 5h. Bovine kidney amino acylase and porcine aminoacylase resolution and D-Ala yield the highest were 90% and 84%; 89.7% and 66.1%, indicating that bovine kidney amino acylase Resolution and yield are better than porcine renal amino acylase.