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目的 :探讨 P53蛋白表达在癌前病变早期定性诊断的价值和在喉癌发生中的作用机理。方法 :应用 P53单克隆抗体 ( DO- 1 )对 1 9例喉癌鳞状细胞癌、34例喉粘膜不典型增生上皮和 1 7例声带息肉进行免疫组织化学染色。结果 :显示 P53蛋白阳性物质位于癌细胞核内和转化的细胞核内。在 1 9例喉癌中 P53蛋白表达阳性者 1 4例 ,表达率为 73.68%;在 34例不典型增生上皮中有2 0例 P53蛋白表达阳性 ,表达率为 58.82 %,在 1 7例声带息肉中未见有 P53蛋白的表达。结论 :P53蛋白免疫组化可以做为临床上癌前病变早期定性诊断和喉癌早期诊断的指标之一
Objective: To investigate the value of P53 protein expression in the early diagnosis of precancerous lesions and its mechanism in the pathogenesis of laryngeal carcinoma. Methods: Immunohistochemical staining was performed on 19 squamous cell carcinomas of the larynx, 34 atypical hyperplasia of the laryngeal mucosa, and 17 vocal cord polyps using the P53 monoclonal antibody (DO-1). Results: The P53 protein positive substance was found in the nucleus of the cancer cells and in the transformed nucleus. In 19 cases of laryngeal carcinoma, P53 protein was positive in 14 cases, the expression rate was 73.68%; in 34 cases of atypical hyperplasia, 20 cases of P53 protein expression was positive, the expression rate was 58.82% in 17 cases of vocal cord P53 protein expression was not observed in polyps. Conclusion: P53 protein immunohistochemistry can be used as an early diagnosis of precancerous lesions in clinical diagnosis of early laryngeal cancer and one of the indicators