论文部分内容阅读
目的:采用反相高效液相色谱法建立金铃子汤中原阿片碱、黄连碱、巴马汀、延胡索乙素、川楝素含量同时测定的方法。方法:采用Kromasil C18(250 mm×4.6 mm,5μm)色谱柱,流动相为乙腈-0.05%醋酸水溶液(氨水调pH 6.0),梯度洗脱,流速0.8mL·min-1,检测波长230 nm,柱温30℃。结果:原阿片碱、黄连碱、盐酸巴马汀、延胡索乙素、川楝素质量浓度分别在1.76～35.2,1.84～36.8,2.20～44.0,2.24～44.8,8.48～63.6μg·mL-1范围内线性良好(r=0.9998～0.9999);平均回收率(n=6)分别为103.5%,101.2%,102.7%,100.9%,97.1%。结论:本方法简单快捷,测定结果准确,适用于金铃子汤中原阿片碱、黄连碱、巴马汀、延胡索乙素、川楝素的含量测定,可为金铃子汤的质量控制或中药配伍等方面给与全面、客观的评价。
OBJECTIVE: To establish a method for the simultaneous determination of protopine, berberine, palmatine, tetrahydropalmatine and toosendanin in Jinlingzi Decoction by reversed-phase high performance liquid chromatography. Methods: A Kromasil C18 (250 mm × 4.6 mm, 5 μm) column was used. The mobile phase consisted of acetonitrile-0.05% acetic acid in water (adjusted to pH 6.0 with ammonia water) and gradient elution at a flow rate of 0.8 mL · min- Column temperature 30 ℃. Results: The concentrations of protopine, berberine, palmatine hydrochloride, tetrahydropalmatine and toosendanin were in the range of 1.76 ~ 35.2,1.84 ~ 36.8,2.20 ~ 44.0,2.24 ~ 44.8,8.48 ~ 63.6μg · mL-1 (N = 6) were 103.5%, 101.2%, 102.7%, 100.9% and 97.1%, respectively. The average recovery was 0.9998 ~ 0.9999. Conclusion: The method is simple and rapid, and the determination results are accurate. It is suitable for the determination of Zhongyuan opium alkaloids, berberine, palmatine, tetrahydropalmatine and toosendanin in Jinlingzi Decoction. It can be used for the quality control of Jinlingzi Decoction or traditional Chinese medicine Compatibility and other aspects to give a comprehensive and objective evaluation.