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随着血清免疫学诊断的迅速发展,双向琼脂扩散试验和对流免疫电泳等免疫学技术,在国内已成为临床上AFP及HBAg等检测的基本方法。上述检测方法皆以琼脂凝胶为载体。由于琼脂凝胶的质量在很大程度上直接关系到实验结果,目前实验室内制备凝胶载体,一般都采用Tris缓冲液及日本琼脂粉,或者是经过纯化的国产琼脂。然而Tris缓冲液的试剂和日本琼脂粉必须依靠进口,国产琼脂的纯化过程亦较麻烦,使得这些基本的免疫学诊断技术的推广和在基层医疗部门的普及造成许多困难。
With the rapid development of serum immunological diagnosis, immunological techniques such as two-dimensional agar diffusion test and convective immunity electrophoresis have become the basic methods for the detection of AFP and HBAg clinically in China. The above detection methods are agar gel as a carrier. Since the quality of agar gel is directly related to the experimental results to a large extent, gel carriers are currently prepared in the laboratory, generally using Tris buffer and agar powder, or purified domestic agar. However, Tris buffer reagent and Japan agar powder must rely on imports, domestic agar purification process is also more troublesome, making the promotion of these basic immunological diagnostic techniques and the popularization of primary health care departments cause many difficulties.