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目的:建立同时测定注射用三花中6种成分的HPLC方法。方法:色谱柱为Discovery C18(250 mm×4.6mm,5μm),流动相为乙腈-0.05%磷酸水溶液(梯度洗脱);检测波长为203 nm;体积流量为1.0 mL/min;柱温为30℃。结果:通过方法学考察,野黄芩苷、三七皂苷R1、人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1、人参皂苷Rd质量浓度分别在0.0116~0.3726 mg/mL、0.0171~0.5466 mg/mL、0.0693~2.2180 mg/mL、0.0094~0.3010 mg/mL、0.0735~2.3527 mg/mL、0.0181~0.5783 mg/mL范围内与峰面积呈良好的线性关系(r≥0.99965),野黄芩苷、三七皂苷R1、人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1、人参皂苷Rd平均回收率(n=9)分别为100.15%、99.43%、100.69%、102.86%、98.99%、99.03%,RSD值分别为1.61%、1.18%、1.63%、1.47%、0.96%、0.86%。结论:该方法简便易行,且能同时测定注射用三花中6种成分的含量,可作为本品多成分内控质量的测定方法。
Objective: To establish an HPLC method for the simultaneous determination of six components in three kinds of flowers for injection. METHODS: The chromatographic column was developed as C18 (250 mm × 4.6 mm, 5 μm) with a mobile phase of acetonitrile-0.05% phosphoric acid (gradient elution). The detection wavelength was 203 nm. The volume flow rate was 1.0 mL / min. ℃. Results: The mass concentration of wild baicalin, notoginsenoside R1, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1 and ginsenoside Rd were 0.0116 ~ 0.3726 mg / mL, 0.0171 ~ 0.5466 mg / mL and 0.0693 ~ 2.2180 mg / mL, 0.0094 ~ 0.3010 mg / mL, 0.0735 ~ 2.3527 mg / mL and 0.0181 ~ 0.5783 mg / mL respectively. The results showed that there was a good linear relationship between the concentrations of wild baicalin and notoginsenoside R1 , The average recoveries of ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1 and ginsenoside Rd were 100.15%, 99.43%, 100.69%, 102.86%, 98.99% and 99.03%, respectively, with the RSDs of 1.61% , 1.18%, 1.63%, 1.47%, 0.96%, 0.86%. Conclusion: The method is simple and easy to operate, and can simultaneously determine the content of 6 kinds of ingredients in three kinds of flowers for injection, which can be used as the method for determining the quality of multi-component internal control of this product.