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目的 探讨用超声微泡造影剂促进VEGF基因治疗兔下肢缺血的可行性。方法 将30 只大白兔左股动脉结扎后随机分为3组: A组采用局部注射VEGF质粒DNA; B组采用局部注射VEGF质粒与超声微泡造影剂的混合物;C组作为对照。于治疗后4周进行数字减影血管造影(DSA)和免疫组织化学方法检测侧支循环建立和血管新生。结果 DSA可见用超声破坏微泡后促进侧支循环建立较好,新生血管较多;单纯局部注射质粒可促进部分血管新生,而对照组的侧支循环和新生血管较少。各组免疫组化所测血管计数均有显著性差异。结论 用超声微泡介导的VEGF基因转染,可促进缺血骨骼肌的血管新生和侧支循环建立,为下肢缺血性疾病的基因治疗提供了一种新途径。
Objective To investigate the feasibility of using ultrasound microbubble contrast medium to promote VEGF gene therapy in rabbit lower limb ischemia. Methods Thirty rabbits were randomly divided into three groups after ligation of the left femoral artery: Group A received local injection of VEGF plasmid DNA; Group B received local injection of a mixture of VEGF plasmid and ultrasound microbubble contrast agent; Group C served as a control. Four weeks after treatment, digital subtraction angiography (DSA) and immunohistochemistry were performed to detect collateral circulation and angiogenesis. Results DSA showed that the use of ultrasound to destroy the microvesicles to promote collateral circulation to establish a better, more new blood vessels; only a local injection of plasmid can promote partial angiogenesis, while the control group collateral circulation and fewer new blood vessels. Each group immunohistochemistry measured blood vessel counts were significantly different. Conclusion Ultrasound-mediated microbubble-mediated VEGF gene transfection can promote angiogenesis and collateral circulation in ischemic skeletal muscle and provide a new approach for gene therapy of ischemic diseases of the lower extremities.