Protein Kinase C Gene Expression in Brain and Heart in Spontaneously Hypertensive Rats

• 作 者: 高平进 赵光胜 袁晓源 赵涵芳 程枫 陈诗书
• 出 处: 高血压杂志
• 发表日期: 1995年03期
• 关键词: Kinase formamide cortex pendent hence RNA

We detected PKC isoform gene expression in the heart and brain of SHR using Northern blot analysis.The resultss revealed significant increased levels of expression of the Ca~(2+)-dependent isoforms PKC-Y in the cerebral cortex of 4 and 20 weeks SHR compared with WKY, while Ca~(2+)-inde pendent isoforms PKC-e did not differ between SHR and WKY.Interestingly，in ventricular myocardium,there was a significant expression of the Ca~(2+)-inde pendent isoform PKC-εin 4 and 20 weeks SHR compared with WKY.Ca~(2+)-dependent PKC-Y may be mainly involved in the modulation of blood pressure in the level of the central nervous system,while Ca~(2+)-independent PKC-εcould be related to the genetic myocardial hypertrophy.

We detected PKC isoform gene expression in the heart and brain of SHR using Northern blot analysis. The resultss revealed significant increased levels of expression of the Ca ~ (2 +) - dependent isoforms PKC-Y in the cerebral cortex of 4 and 20 weeks SHR Compared with WKY, while Ca ~ (2 +) - inde pendent isoforms PKC-e did not differ between SHR and WKY.Interestingly, in ventricular myocardium, there was a significant expression of the Ca ~ (2 +) - inde pendent isoform PKC -εin 4 and 20 weeks SHR compared with WKY.Ca ~ (2 +) - dependent PKC-Y may be mainly involved in the modulation of blood pressure in the level of the central nervous system, while Ca ~ (2 +) - independent PKC-εcould be related to the genetic myocardial hypertrophy.