论文部分内容阅读
目的探讨牛磺酸对染锰大鼠神经氧化损伤的影响。方法选取Wistar大鼠45只,随机分为对照组、染锰组和牛磺酸组,每组各15只。对照组腹腔注射(ip)生理盐水30 mg/kg,每日1次,共20次;染锰组ip注射15 mg/kg MnCl_2·4H_2O溶液,1次/d,共20次;牛磺酸组在染锰组基础上,皮下注射牛磺酸125.15 mg/kg,隔日1次共注射10次。进行Morris水迷宫试验、跳台试验,分别检测大鼠丘脑中谷氨酰胺酶、谷氨酸脱羧酶、谷氨酰胺合成酶含量,超氧化物歧化酶(SOD)、丙二醛(MDA)含量。结果染锰组与对照组比较,染锰组SOD明显降低[(35.57±3.26)μg/L]、MDA含量明显增高[(230.32±35.28)nmol/g],Morris水迷宫的潜伏期[(41.2±3.1)s)明显延长、错误次数[(5.4±2.5)次]明显增加,跳台试验潜伏期第1天[(55.6±7.8)s]、第2天[(49.7±9.3)s]、第3天[(42.8±8.7)s]、第4天[(35.7±8.4)s]均明显延长,谷氨酰胺酶C(0.72±0.08)μg/L]、谷氨酸脱羧酶[(107.64±8.54)ng/L]、谷氨酰胺合成酶[(142.68±28.96)ng/L]含量均明显降低,差异有统计学意义(P<0.01)。牛磺酸组与染锰组比较,牛磺酸组SOD[(39.47±4.15)μg/L]含量明显增高,MDA含量[(203.05±32.53)nmol/g]明显降低,Morris水迷宫潜伏期[(30.5±2.9)s]明显缩短,错误次数[(3.9±1.4)次]明显减少,跳台试验潜伏期第1天[(46.7±7.4)s)、第2天[(38.6±8.9)s]、第3天[(33.7±7.8)s]、第4天[(27.6±6.9)s]均明显缩短,谷氨酰胺酶[(0.82±0.09)μg/L]、谷氨酸脱羧酶([(135.36±11.79)ng/L]、谷氨酰胺合成酶[(168.73±29.97)ng/L]含量均明显增高,差异均有统计学意义(P<0.01)。结论牛磺酸明显提高了染锰大鼠的学习记忆能力,改善了脑组织谷氨酸代谢,抑制了神经过氧化反应,对染锰大鼠神经氧化损伤具有保护作用。
Objective To investigate the effect of taurine on oxidative damage of manganese in rats. Methods Forty-five Wistar rats were randomly divided into control group, manganese group and taurine group, 15 rats in each group. The control group was intraperitoneally injected with normal saline (30 mg / kg) once a day for 20 times. The manganese-exposed group was injected with 15 mg / kg MnCl 2 .H 4 O 2 solution once a day for 20 times. The taurine group On the basis of Mn-dyed group, taurine 125.15 mg / kg was injected subcutaneously and co-injected 10 times every other day. Morris water maze test and platform test were performed to detect the levels of glutaminase, glutamate decarboxylase, glutamine synthetase, superoxide dismutase (SOD) and malondialdehyde (MDA) in rat thalamus. Results Compared with the control group, the content of MDA in the manganese-treated group was significantly lower ([(35.57 ± 3.26) μg / L] and the MDA content was significantly higher in the manganese-exposed group [(230.32 ± 35.28) nmol / g] 3.1) s) was significantly prolonged, the number of errors was significantly increased [(5.4 ± 2.5) times], the first day of jumping test was (55.6 ± 7.8) s, the second day was (49.7 ± 9.3) s, (42.8 ± 8.7) s] and 4 days [(35.7 ± 8.4) s], respectively. Glutamine C (0.72 ± 0.08) μg / L] and glutamate decarboxylase [(107.64 ± 8.54) ng / L] and glutamine synthetase [(142.68 ± 28.96) ng / L], all of which were statistically significant (P <0.01). The content of SOD [(39.47 ± 4.15) μg / L] in taurine group was significantly higher than that in the group of taurine, the MDA content [(203.05 ± 32.53) nmol / g] 30.5 ± 2.9 s) was significantly shortened and the number of errors (3.9 ± 1.4) was significantly decreased. The latency of the test on the first day was (46.7 ± 7.4) s on day 1, (38.6 ± 8.9 s) on day 2, 3 days (33.7 ± 7.8) s and 4 days (27.6 ± 6.9 s), respectively. The levels of glutaminase (0.82 ± 0.09 μg / L) and glutamate decarboxylase (135.36 ± 11.79 ng / L] and glutamine synthetase [(168.73 ± 29.97) ng / L], all of which were statistically significant (P0.01) .Conclusion Taurine can significantly increase the content of Mn The learning and memory abilities of rats improved glutamate metabolism in brain tissue, inhibited neuro-peroxidation and had protective effect on oxidative damage of oxidative damage in rats with manganese.