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设计、合成了一种新的蛋白质特异性氧化断裂试剂BAPBE，用它共价修饰BSA的唯一疏基．修饰物与Fe2+螯合，在pH7．0、25℃下用H2O2及抗坏血酸钠处理，使BSA发生2种方式的断裂，产生4种BSA片段，但在相同条件下巯基枯草杆菌蛋白酶未被切断．
A new protein-specific oxidative cleavage reagent, BAPBE, was designed and synthesized to covalently modify the unique spinel of BSA. The modifier chelated with Fe2 +, treated with H2O2 and sodium ascorbate at pH 7.0 at 25 ° C, broke the BSA in two ways, yielding four BSA fragments, but the thiol subtilisin was not cleaved under the same conditions.