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目的 :研究异丙酚、咪达唑仑对交感神经节细胞钠通道电流的联合作用 ,探讨两者复合诱导时循环稳定的可能机制。方法 :全细胞膜片钳技术记录急性分离SD大鼠 (7～ 10d)颈上交感神经节细胞钠通道电流 ;等辐射线图法分析异丙酚、咪达唑仑复合对交感神经节细胞钠通道电流的影响。结果 :在钳制电压 (Vh) 80mV、刺激电压 (Vt) 0mV条件下 ,临床相关浓度的异丙酚、咪达唑仑分别使钠通道电流峰值降低 2 7.6 6 % (P <0 .0 1)和 19.98% (P <0 .0 5 ) ,随浓度增加 ,抑制作用逐渐增强 ,5 0 %的钠通道电流受抑制时的浓度 (IC50 )分别为 33.12 μmol·L- 1和18.35 μmol·L- 1。 1/4、3/4IC50 的异丙酚与咪达唑仑合用时的IC50 数值点落在理论上的相加等效线的95 %可信区间内 ;1/2IC50 的异丙酚与咪达唑仑合用时的IC50 数值点落在理论上的相加等效线的 95 %可信区间的左下方。结论 :临床相关浓度的异丙酚、咪达唑仑对交感神经节细胞钠通道电流有明显的抑制作用 ,且呈浓度依赖性 ;除等效剂量合用呈协同抑制作用外 ,两者复合主要呈相加抑制交感神经节细胞钠通道流 ;复合诱导时循环稳定可能与两者催眠作用明显协同导致诱导剂量的降低 ,从而减弱对交感神经的抑制有关
OBJECTIVE: To investigate the combined effects of propofol and midazolam on sodium channel currents in sympathetic ganglion cells and to explore the possible mechanism of the cyclic stability of the compound induced by propofol and midazolam. Methods: The whole-cell patch-clamp technique was used to record the sodium channel currents in the sympathetic ganglion of the cervical spine isolated from acutely isolated SD rats (7-10 days). The isoflurane and midazolam combined with sodium dodecyl sulphate Effect of current. Results: The clinically relevant concentrations of propofol and midazolam reduced the peak currents of sodium channels by 2 7.66% (P <0.01) at clamping voltage (Vh) of 80 mV and stimulating voltage (Vt) of 0 mV, respectively. And 19.98% (P <0. 05), respectively. With increasing concentration, the inhibitory effect was gradually enhanced. The IC50 values of 50% sodium currents were 33.12 μmol·L-1 and 18.35 μmol·L- 1. IC50 values for the 1 / 4,3 / 4IC50 combination of propofol and midazolam fell within a 95% confidence interval for the theoretical additive equivalence line; 1 / 2IC50 of propofol and mida The IC50 values for azole in combination fall to the bottom left of the 95% confidence interval for the theoretical additive equivalence line. CONCLUSION: Propofol and midazolam in clinically relevant concentrations have a significant inhibitory effect on the sodium channel current in sympathetic ganglion cells in a concentration-dependent manner. The combination of propofol and midazolam in a dose-dependent manner has a synergistic effect. Synergistic inhibition of sympathetic ganglion cells sodium channel flow; complex induction induced by cyclic hypothyroidism may be significantly synergistic with the reduction of induced dose, thus reducing the inhibition of sympathetic