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目的 探讨单次腹腔注射脂多糖 (LPS) ,中脑和桥脑神经元、星形胶质细胞和小胶质细胞的可塑性变化。方法 抗Fos蛋白、抗胶质原纤维酸性蛋白 (GFAP)、抗特异性标记小胶质细胞 (OX4 2 )和抗Fos/GFAP双重免疫组织化学标记法。结果 Fos阳性神经元分布于上丘、中脑导水管周围灰质、臂旁核和蓝斑。Fos蛋白在注射后 30min表达 ,1～ 3h为高峰。GFAP阳性星形胶质细胞胞体变大 ,突起增粗 ,细胞密度增加 ,30min出现表达 ,1h为高峰 ,3h后减少。 0X4 2阳性小胶质细胞首先于脑室周围灰质表达 ,注射后 6h达到高峰 ,胞体变大 ,全脑分布。相应于Fos阳性神经元分布区域 ,GFAP阳性星形胶质细胞和OX4 2阳性小胶质细胞深染和密集。结论 上丘、臂旁核、蓝斑内神经元、星形胶质细胞、小胶质细胞可能参与神经免疫调节 ,臂旁核可能为此调节通路的中继站之一。
Objective To investigate the plasticity of single intraperitoneal injection of lipopolysaccharide (LPS), midbrain and pons cerebral neurons, astrocytes and microglia. Methods Anti-Fos protein, anti-glial fibrillary acidic protein (GFAP), anti-specific marker microglia (OX4 2) and anti-Fos / GFAP dual immunohistochemical method. Results Fos positive neurons were distributed in the superior colliculus, midbrain periaqueductal gray matter, parabrachial nucleus and locus coeruleus. Fos protein expression 30min after injection, 1 ~ 3h for the peak. GFAP positive astrocytes became larger, the protrusions thicker, cell density increased, 30min expression, 1h peak, decreased after 3h. 0X4 2-positive microglial cells were first expressed in the periventricular gray matter, and reached the peak at 6h after injection. The cell body became larger and the whole brain was distributed. The GFAP-positive astrocytes and OX4 2-positive microglial cells were deeply stained and dense corresponding to the region of Fos-positive neuron distribution. Conclusion The superior colliculus, parabrachial nucleus, intracortical neurons, astrocytes and microglial cells may be involved in neuroimmunoregulation. Parabrachial nuclei may be one of the relay stations for this regulatory pathway.