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探讨海马神经元损害的机理及丹参的保护作用．方法：作者检测了大鼠局限性脑皮质损伤后海马ＣＡ－１区组织腺苷三磷酸（ＡＴＰ）酶活性，Ｎａ＋，Ｋ＋，Ｃａ＋离子含量，并观察了病理组织学的改变．结果：脑损伤后海马组织Ｎａ＋－Ｋ＋－ＡＴＰ酶、Ｃａ２＋－ＡＴＰ酶活性分别为０．４２５±０．０６６和０．４１９±０．０２０μｍｏｌＰｉ／（ｍｇ蛋白·ｈ），较正常对照组（Ｎａ＋－Ｋ＋－ＡＴＰ酶０．６３５±０．０４３，Ｃａ２＋－ＡＴＰ酶０．５２７±０．０１７）明显降低（Ｐ＜０．０１）；Ｎａ＋，Ｃａ２＋含量（Ｎａ＋２１５．７４±２４．３０，Ｃａ２＋４．５９±０．２５μｍｏｌ／ｇ干脑）较正常对照组（Ｎａ＋１４２．２５±１１．３９，Ｃａ２＋３．７３±０．３８）明显增高（Ｐ＜０．０１）．丹参治疗组大鼠海马组织ＡＴＰ酶活性抑制（Ｎａ＋－Ｋ＋－ＡＴＰ酶０．５９３±０．０２７，Ｃａ２＋－ＡＴＰ酶０．４６８±０．０４０）及Ｎａ＋，Ｃａ２＋聚积（Ｎａ＋１８１．７２±１９．６２，Ｃａ２＋４．０８±０．３８）程度较脑损伤组显著降低（Ｐ＜０．０１），电镜观察神经元损害也明显减轻．结论：提示丹参可能通过改善ＡＴＰ酶功能、抑制Ｎａ＋，Ｃａ２＋聚积而减轻脑损伤后海?
To explore the mechanism of hippocampal neuronal damage and the protective effect of Salvia miltiorrhiza. METHODS: The authors examined the tissue adenosine triphosphate (ATP) enzyme activity, Na+, K+, Ca+ ion content in hippocampal CA-1 region after focal cerebral cortex injury in rats, and observed histopathological changes. Results: The activities of Na+-K+-ATPase and Ca2+-ATPase in the hippocampus after brain injury were 0.425±0.066 and 0.419±0.020μmolPi/(mg protein·h), respectively, compared with the normal control group (Na+ -K+-ATPase 0.635±0.043 and Ca2+-ATPase 0.527±0.017 were significantly decreased (P<0.01); Na+ and Ca2+ contents were (Na+215.74±24.30, Ca2+4. 59±0.25μmol/g dry brain was significantly higher than normal control group (Na+142.25±11.39, Ca2+3.73±0.38) (P<0.01). The inhibition of ATPase activity in hippocampal tissue of rats treated with Salvia Miltiorrhiza (Na+-K+-ATPase 0.593±0.027, Ca2+-ATPase 0.468±0.040) and Na+, Ca2+ accumulation (Na+181.72±19. 62, Ca2+4.08±0.38) were significantly lower than those in brain injury group (P<0.01), and electron microscope observation of neuronal damage was also significantly reduced. Conclusion: It is suggested that salvia miltiorrhiza can reduce sea damage after brain injury by improving ATPase function and inhibiting accumulation of Na+ and Ca2+.