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目的:建立人眼虹膜色素上皮细胞体外培养并对其进行冻存与复苏。方法:直接刮取虹膜色素上皮细胞组织碎屑进行培养,光镜观察生长特性及形态特点,透射电镜观察其超微结构。根据慢冻速融的细胞冻存原则,定期收集细胞进行液氮冻存,至少2个月后进行复苏。结果:人眼虹膜色素上皮细胞体外培养成功,原代细胞在光镜下呈多角形单层生长,胞浆内有丰富的色素颗粒;电镜下见胞浆富含色素、细胞器丰富、细胞膜有明显微绒毛、微丝、相临细胞之间可见桥粒连接。共冻存6批细胞,进行4次复苏实验均成功,每次复苏细胞存活为90％。结论:人眼虹膜色素上皮细胞体外培养的建立及冻存、复苏的成功,为研究某些疾病提供有利的基础。眼科学报2000;16:220～223。
Objective: To establish a human iris pigment epithelial cell culture in vitro and cryopreservation and resuscitation. Methods: Iris pigment epithelial cells were directly scraped for culturing. The growth characteristics and morphological characteristics were observed under light microscope. The ultrastructure of the cells was observed by transmission electron microscope. According to the principle of cryo-thawing, the cells are collected regularly for liquid nitrogen cryopreservation and resuscitated after at least 2 months. Results: Human iris pigment epithelial cells were cultured successfully in vitro. The primary cells grew in polygonal monolayer under light microscope. There were abundant pigment granules in the cytoplasm. Under the electron microscope, the cytoplasm was rich in pigment, abundant organelles and obvious cell membrane Microvilli, Microfilaments, adjacent cells seen desmosomes. A total of six batches of cells were cryopreserved, and four resuscitation experiments were successful. The survival of each resuscitation cell was 90%. Conclusion: The establishment of human iris pigment epithelial cells in vitro and the success of cryopreservation and resuscitation provide a good foundation for the study of some diseases. Journal of Ophthalmology 2000; 16: 220 ~ 223.