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目的:观察具有食疗保健作用的脱糖南瓜粉对预防和治疗四氧嘧啶糖尿病小鼠血糖值升高的作用。方法:实验于2003-03/06在浙江省医学科学院营养与食品卫生研究室完成。①预防高血糖实验:40只小鼠雌雄兼用随机分为实验组和对照组,每组20只。实验期8d,按剂量灌胃,1次/d,对照组灌蒸馏水,实验组灌脱糖南瓜粉10g/kg体质量,自由进食、饮水。在实验第4d,小鼠禁食16h,对照组和实验组均按200mg/kg剂量腹腔注射四氧嘧啶生理盐水液,72h后测空腹血糖和体质量。②降血糖实验:取雌性小鼠60只,称重,禁食16h,按200mg/kg剂量腹腔注射四氧嘧啶生理盐水液,72h后测空腹血糖,取血糖值在15～30mmol/L的小鼠30只,按血糖值、体质量随机分为对照组和实验组,每组15只。按剂量灌胃1次/d,对照组灌蒸馏水,实验组灌脱糖南瓜粉液10g/kg体质量,小鼠自由进食、饮水,记录饮水量,称质量1次/周。实验期为2周,在实验末期测小鼠空腹血糖值、体质量及饮水量。结果:进入结果分析的小鼠为70只。①预防高血糖试验结果:小鼠空腹血糖实验组明显低于对照组犤(17.4±8.6,28.3±6.1)mmol/L,(P<0.05)犦;实验末期小鼠体质量实验组明显高于对照组犤(25.9±2.9,19.9±1.3)g,(P<0.001)犦。②降血糖试验结果:实验末期小鼠血糖对照组明显高于实验组犤(18.9±7.1,12.1±5.6)mmol/L,(P<0.01)犦;体质量有一定的回升,实验组小鼠比对照组略高,但其差别无显著性意义;饮水量实验组明显低于对照组犤(12.5±3.9,26.3±1.1)mL,(P<0.001)犦。结论:脱糖南瓜粉具有明显预防四氧嘧啶糖尿病小鼠血糖值升高、体质量减轻的作用;并且具有降低四氧嘧啶糖尿病小鼠空腹血糖值、控制其饮水量、缓解烦渴症状的作用。
OBJECTIVE: To observe the effect of dehydrated squash powder with dietary health care on the prevention and treatment of elevated blood glucose levels in alloxan-induced diabetic mice. METHODS: The experiment was performed at the Nutrition and Food Hygiene Lab of Zhejiang Academy of Medical Sciences from March 2003 to June 2006. 1 Prevention of hyperglycemia: 40 male and female mice were randomly divided into experimental and control groups, with 20 mice in each group. During the experimental period of 8 days, gavage was performed once a day for one time. The control group was filled with distilled water. The experimental group was poured with 10g/kg body weight of pumpkin powder, and was allowed to eat freely and drink water. On the 4th day of the experiment, the mice fasted for 16 h, the control group and the experimental group received an intraperitoneal injection of alloxan physiological saline at a dose of 200 mg/kg, and the fasting blood glucose and body weight were measured after 72 hours. 2 Hypoglycemic experiment: Take 60 female mice, weigh, fast for 16h, according to 200mg/kg dose of intraperitoneal injection of alloxan saline, 72h after fasting blood glucose, blood glucose value of 15 ~ 30mmol / L Thirty mice were randomly divided into a control group and an experimental group according to the blood glucose level and body mass, and 15 rats in each group. Gavage once a day by volume, the control group was irrigated with distilled water, and the experimental group was administrated with 10g/kg body weight of pumpkin powder. The mice were allowed to eat freely and drink water. The amount of drinking water was recorded and the quality was once/week. The experimental period was 2 weeks. At the end of the experiment, the fasting blood glucose, body weight and drinking water were measured. Results: There were 70 mice that entered the results analysis. 1 Prevention of hyperglycemia test results: The experimental group of fasting blood glucose in mice was significantly lower than that in the control group (17.4±8.6, 28.3±6.1) mmol/L, (P<0.05); the mice in the experimental group at the end of the experiment were significantly higher than those in the experimental group. The control group was (25.9±2.9, 19.9±1.3) g, (P<0.001). 2 Hypoglycemic test results: At the end of the experiment, the mice in the blood glucose control group were significantly higher than those in the experimental group (18.9±7.1, 12.1±5.6) mmol/L, (P<0.01); there was a certain recovery in the body weight, and the mice in the experimental group Slightly higher than the control group, but the difference was not significant; the drinking water experimental group was significantly lower than the control group (12.5±3.9, 26.3±1.1) mL, (P<0.001). Conclusion: De-sugared pumpkin powder can significantly prevent the increase of blood glucose and body weight of alloxan-induced diabetic mice, and it can reduce the fasting blood glucose level, control the drinking water and relieve the symptoms of polydipsia in alloxan-induced diabetic mice. .