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目前国内外常用测定斯氏线虫对Sleinernema spp.寄主侵染力的方法是沙埋法(Bedding 1983),把目标昆虫埋入一装有含水量为7％的80克细沙的标准盒底部,沙上滴加线虫悬浮液。对非土栖昆虫,是不适宜的。York(1957)在测定DD-136线虫对欧洲玉米螟(ECB)的侵染力时,采用培养皿底部放滤纸,再加入线虫液和接入ECB,但由于ECB的逃逸而使试验失败。后来他在培养皿里加鲜玉米组织,才使试验成功。作者在研究斯氏线虫对亚洲玉米螟(ACB)侵染力的过程中,设计了一种兼有密封性、透气性,并能满足昆虫生存与习性要求的测试方法,现介绍如下。
At present, the commonly used method to determine host infection of Sleinernema spp. At home and abroad is the sand-burying method (Bedding 1983), and the target insects are buried in the bottom of a standard box containing 80 g of fine sand with a water content of 7% Sand on the nematode suspension. For non-native insects, is not appropriate. York (1957) When measuring the infectivity of the DD-136 nematode to the European corn borer (ECB), York (1957) placed the filter paper on the bottom of the dish, added nematode fluid and inserted into the ECB, but the test failed due to ECB escape. Later, he was in Petri dish fresh corn organization, the trial was successful. In the study of the infection of Asiatic to the Asian Corn Borer (ACB), the authors designed a test method that combines both sealability and air permeability, and can meet the requirements of insect survival and habits.