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目的 获得不易感动脉粥样硬化动物树胆固醇酯转运蛋白 (CETP)的cDNA和蛋白质序列 ,分析其结构特点 ,寻找其可能的抗动脉粥样硬化分子机制。方法 以从树肝脏mRNA反转录获得的cDNA一链为模板 ,应用SMART RACE技术 ,获得了树CETP的cDNA序列 ,并推导出其蛋白质氨基酸序列 ,应用分子生物学软件对该蛋白的一级、二级结构进行分析和比较。结果 获得的树CETPcDNA(在GenBank中的注册号为AF334 0 33)长 16 36bp ,编码 485个氨基酸 ,其成熟蛋白由 477个氨基酸组成 ,比人多一个氨基酸 (Gly318) ,该蛋白与人、家兔的同源性分别为 88%和 82 %。序列中与CETP结合和转运中性脂质功能相关的区域均非常保守 ,但在Asn34 2位的N 糖基化位点缺失 ,可能使其转运胆固醇酯的活性增加 ,利于外周组织和血浆中的胆固醇及其酯的清除。通过对不同种属蛋白序列的比较 ,初步分析了树CETP蛋白与功能相关的结构特点。结论 树CETP糖基化的特点有可能是该动物对动脉粥样硬化具有不易感性的分子机理之一
Objective To obtain the cDNA and protein sequence of CETP in the atherosclerotic animal, and to analyze the structural features of CETP and to find out its possible anti-atherosclerosis molecular mechanism. Methods The cDNA sequence of reverse transcription of the liver mRNA of tree shrews was used as a template. The cDNA sequence of the shrew cDNA was obtained by using SMART RACE technique. The amino acid sequence of the protein was deduced. Level, secondary structure analysis and comparison. The results obtained tree CETPcDNA (GenBank accession number AF334 033) length 1636bp, encoding 485 amino acids, the mature protein consists of 477 amino acids, one more than human (Gly318), the protein and human, Homology in rabbits was 88% and 82%, respectively. The sequence of CETP binding and transport neutral lipid function are very conservative, but the lack of N glycosylation site in Asn34 2 position may increase its cholesterol ester transport activity, which is conducive to peripheral tissues and plasma Clearance of cholesterol and its esters. Through the comparison of the protein sequences of different species, the structural characteristics of the protein related to function of tree CETP were preliminarily analyzed. CONCLUSIONS: The glycosylation of tree CETP is likely to be one of the molecular mechanisms underlying the atherosclerosis in this animal