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目的检测长、短时受精受精液中活性氧的不同,分析精卵孵育不同时间对体外受精-胚胎移植结局的影响。方法 155例接受常规IVF受精的患者,每人取出的OCCC随机分为两组:A组为短时受精,B组为长时受精。检测受精液中H2O2、CTA的含量。移植的119名患者随机分为两组,一组60人移植短时受精的胚胎,一组59人移植长时受精的胚胎。结果 B组的受精液中H2O2水平明显高于A组(P<0.05),CTA水平明显低于A组(P<0.05)。B组的多精受精率明显高于A组(P<0.05),A组的优胚率明显高于B组(P<0.05),两组间的受精率、卵裂率、植入率、临床妊娠率无统计学差异。结论长时受精时,过量的活性氧对卵子及胚胎有不利影响。短时受精在不影响受精率的前提下,可以降低多精受精率,提高优胚率。
Objective To detect the differences of reactive oxygen species (ROS) in fertilized sperm between long and short fertilization, and to analyze the effects of different incubation time on the outcome of in vitro fertilization and embryo transfer. Methods A total of 155 patients undergoing IVF fertilization were randomly divided into two groups: A group with short-term fertilization and B group with long-term fertilization. Detection of semen in the H2O2, CTA content. Transplanted 119 patients were randomly divided into two groups, a group of 60 transplanted short-fertilized embryos, a group of 59 transplanted long fertilized embryos. Results The level of H2O2 in the semen of group B was significantly higher than that of group A (P <0.05). The level of CTA in group B was significantly lower than that in group A (P <0.05). The fertilization rate in group B was significantly higher than that in group A (P <0.05). The excellent embryo rate in group A was significantly higher than that in group B (P <0.05). The fertilization rate, cleavage rate, implantation rate, Clinical pregnancy rate was no significant difference. Conclusions Excessive ROS negatively affects eggs and embryos when fertilized for long periods of time. Short-term fertilization does not affect the fertilization rate under the premise, you can reduce the fertilization rate and improve the excellent embryo rate.