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首次以仿生聚多巴胺膜为功能基底膜并结合使用纳米金,构建了一种高导电性、稳健的酶生物分子固定化平台.以固定辣根过氧化物酶(HRP)为例,发展了一种新的电化学酶传感器用于H2O2的测定.结果表明,酶传感器借助聚多巴胺膜对基底电极的高结合力及其高生物亲和性与电活性,并协同纳米金的“电子通道”作用,不仅可以实现酶分子在电极表面的大量而高活性的固定化,而且能促进电子在酶活性中心和电极表面间的快速传递.与采用其它常见聚合物材料(例如壳聚糖)的酶传感器比较,以聚多巴胺/纳米金固定化平台发展的酶传感器具有更优良的检测H2O2的性能.其对H2O2的检测线性范围为4.0×10-7～4.5×10-4mol·L-1,检测限为3.7×10-7mol·L-1,灵敏度为100.2μA·L·mmol-1.此外,该酶传感器还具有优良的检测重现性和存贮稳定性,以及较好的抗干扰能力.
For the first time, a biomimetic immobilization platform with high conductivity and robustness was constructed by using biomimetic dopamine film as the functional basement membrane and gold nanoparticles in combination with immobilized horseradish peroxidase (HRP) as an example A new electrochemical enzyme sensor was used for the determination of H2O2.The results showed that the enzyme sensor based on the high binding force of the dopamine film to the substrate electrode and its high biological affinity and electrical activity and cooperating with the nano- "Can not only achieve a large amount of high activity immobilization of enzyme molecules on the electrode surface but also promote the rapid transfer of electrons between the enzyme activity center and the electrode surface.Compared with other common polymer materials Compared with the enzyme sensor, the enzyme sensor developed by the polydopamine / nano-gold immobilization platform has a better H2O2 detection performance.The detection limit of H2O2 is 4.0 × 10-7 ~ 4.5 × 10-4mol·L-1, The detection limit was 3.7 × 10-7mol·L-1, the sensitivity was 100.2μA · L · mmol-1. In addition, the enzyme sensor also has excellent detection reproducibility and storage stability, and better anti-interference ability .