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目的 探讨膀胱癌中凝集素受体的分布与其分化程度、浸润深度、转移和预后的关系。方法 应用生物素标记的花生凝集素 (PNA)、麦胚凝集素 (WGA)、扁豆凝集素 (LCA)、刀豆凝集素 (ConA)及菜豆凝集素 (PHA)等 5种凝集素对 5 2例人体膀胱癌 ,10例正常人体膀胱粘膜进行亲合组织化学法研究。结果 发现正常膀胱粘膜PNA、WGA及PHA受体阴性 ,PNA受体阳性率随膀胱癌病理分级的上升而递增 ,相互之间差异具有显著性 (P <0 0 5 )。PNA、WGA及PHA受体阳性率在浸润性肿瘤中明显高于浅表性肿瘤 (P <0 0 5 )。 84 0 %淋巴结转移癌与其原发癌的凝集素标记的量上有差异 ,并且与原发肿瘤获得更多的PNA及PHA受体。术后 2a以上复发的膀胱癌中 ,PNA受体的阳性率小于 2a以内复发的膀胱癌中该受体的阳性率 (P <0 0 5 )。结论 PNA、WGA及PHA受体阳性率与膀胱癌浸润深度有关。PNA及PHA与淋巴结转移癌有关。PNA与膀胱癌分化程度有关 ,肿瘤组织内的PNA受体可作为膀胱癌术后预测预后的参考指标之一。
Objective To investigate the relationship between the distribution of lectin receptor and the degree of differentiation, depth of invasion, metastasis and prognosis in bladder cancer. Methods Five lectins including peanut lectin (PNA), wheat germ agglutinin (WGA), lentil lectin (LCA), bean curd lectin (ConA) and bean curd lectin (PHA) Cases of human bladder cancer, 10 cases of normal human bladder mucosa for the study of affinity histochemistry. Results The positive rates of PNA, WGA and PHA receptors in normal bladder mucosa were negative, and the positive rates of PNA receptor were increased with the pathological grade of bladder cancer. The difference was significant (P <0.05). The positive rates of PNA, WGA and PHA receptors in invasive tumors were significantly higher than those in superficial tumors (P <0.05). 84 0% There is a difference in the amount of lectin tagged with its primary cancer in lymph node metastases, and more PNA and PHA receptors are obtained with the primary tumor. The positive rate of PNA receptor in bladder cancer recurrence above 2a was less than that in bladder cancer recurrence after 2a (P <0.05). Conclusion The positive rates of PNA, WGA and PHA receptors are related to the depth of invasion of bladder cancer. PNA and PHA and lymph node metastasis. PNA and bladder cancer related to the degree of differentiation, PNA receptor within the tumor tissue can be used as one of the prognostic indicators of bladder cancer prognosis.