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背景:肿瘤复发是残存的肿瘤细胞重新增殖生长为病灶的过程,在此过程中某些基因可能起着重要的调控作用。目的:探讨E-cadherin,CD44H和VEGF基因的表达与鼻咽癌患者复发及康复的关系,探索促进康复的途径。设计:以诊断为依据,标准对照横断面回顾性研究。地点和对象:本研究由汕头市中心医院耳鼻咽喉-头颈外科完成,选择1995年3月～10月的原发性鼻咽癌放疗的62例鼻咽癌患者,年龄17～70岁。方法:免疫组化SP法检测鼻咽癌组织中E-cadherin,CD44H和VEGF的表达强度。以细胞浆内或细胞膜上出现黄色染色为阳性标记,采用半定量方法进行结果判定。主要观察指标:鼻咽癌组织中E-cadherin,CD44H和VEGF的表达强度。结果:在鼻咽癌放疗后复发组中,E-cadherin的表达减弱(阳性表达率为52.4%),CD44H和VEGF的表达增强(阳性表达率分别为85.7%,80.9%),无复发组中三者阳性表达率分别为85.4%,65.9%,73.2%,两组的表达差异均有显著性意义(P<0.05,P<0.01,P<0.01),CD44H与VEGF在鼻咽癌放疗后复发组中的表达有正相关关系(r=0.535,P<0.05)。结论:E-cadherin,CD44H和VEGF的表达均与鼻咽癌放疗后复发的关系密切,CD44H和VEGF存在协同作用,他们可作为评估复发风险的生物学标志,可预测鼻咽癌患者放疗后康复的效果。
BACKGROUND: Tumor recurrence is the process by which remnant tumor cells proliferate and proliferate into lesions. In this process, some genes may play an important regulatory role. Objective: To investigate the relationship between the expression of E-cadherin, CD44H and VEGF and the relapse and rehabilitation of patients with nasopharyngeal carcinoma (NPC), and to explore the ways to promote rehabilitation. Design: Based on diagnosis, standard cross-sectional cross-sectional retrospective study. Location and Subjects: This study was performed by otolaryngology-head and neck surgery department of Shantou Central Hospital. Sixty-two patients with nasopharyngeal carcinoma undergoing primary nasopharyngeal carcinoma radiotherapy from March to October 1995 were selected, aged 17-70 years. Methods: Immunohistochemical SP method was used to detect the expression of E-cadherin, CD44H and VEGF in nasopharyngeal carcinoma. The cytoplasm or cell membrane yellow stain positive mark, using semi-quantitative determination of the results. MAIN OUTCOME MEASURES: Expression intensity of E-cadherin, CD44H and VEGF in nasopharyngeal carcinoma. Results: The expression of E-cadherin (52.4%) and the expression of CD44H and VEGF in nasopharyngeal carcinoma recurrence group (85.7% and 80.9%, respectively) were significantly lower than those in non-recurrence group The positive expression rates of CD44H and VEGF were 85.4%, 65.9%, 73.2% respectively in the two groups (P <0.05, P <0.01, P <0.01) The expression in the group had a positive correlation (r = 0.535, P <0.05). Conclusion: The expressions of E-cadherin, CD44H and VEGF are closely related to the recurrence of nasopharyngeal carcinoma after radiotherapy. CD44H and VEGF have a synergistic effect. They can be used as biomarkers to evaluate the risk of recurrence, and can predict the prognosis of patients with nasopharyngeal carcinoma after radiotherapy Effect.