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本研究以魔芋不同外植体为实验材料,建立了魔芋离体培养快速繁殖的再生体系,为以后魔芋基因工程进行品质改良奠定了基础。以不同基因型魔芋为实验材料,研究魔芋不同外植体在不同的培养基中诱导不定芽再生的频率。魔芋不同基因型外植体在含适宜6BA、NAA等激素的培养基中形成愈伤组织的难易程度不同,愈伤组织诱导形成不定芽的能力强弱也不一样;不同基因型魔芋外植体的不定芽诱导率最高的是块茎>幼叶>茎段;对魔芋的块茎、茎段和幼叶来说最适合的培养基配方是MS+6-BA 2.5 mg/L+NAA 0.05 mg/L,不同培养基诱导不定芽的能力是块茎>茎段>幼叶,其中,魔芋块茎和茎段的再生能力较强。最合适的生根培养基是MS+1.0 NAA。本研究成功的建立了魔芋不同外植体离体培养再生途径,并获得再生植株。
In this study, different explants of konjac were used as experimental materials to establish rapid regeneration system of konjac in vitro, which laid the foundation for the future improvement of konjac genetic engineering. Different genotypes of konjac were used as experimental materials to study the frequency of adventitious buds regeneration induced by different explants of konjac in different media. Different genotypes of Amorphophallus explants in the appropriate 6BA, NAA and other hormones in the medium of callus formation of different degrees of difficulty, callus induction of the formation of adventitious buds of the strength is not the same; different genotypes of konjac explants The best induction rate of adventitious buds was tuber> young leaves> stem; the most suitable culture medium for tubers, stems and leaves of konjac was MS + 6-BA 2.5 mg / L + NAA 0.05 mg / L, the ability of different medium to induce adventitious buds is tuber> stem> young leaves, among them, konjac tuber and stem regeneration ability. The most suitable rooting medium is MS + 1.0 NAA. In this study, we successfully established a regeneration pathway in vitro of different explants of Konjac and obtained regenerated plants.