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目的 探讨外周血有核细胞中白蛋白 (Alb)基因在肝癌诊断和转移监测中的价值。方法 以逆转录巢式PCR法扩增肝癌组织及外周血中Alb基因片段 ,分析其在肝癌诊断和转移监测中的价值。结果 2 0份肝癌、癌周和远癌组织总RNA浓度 ,表现为远癌和癌周组织明显高于癌灶组织 (P <0 0 5 )。Alb基因检出率在所有肝组织均为 10 0 % ,在肝癌患者外周血中为 5 9 7%。明显高于肝硬化、慢性肝炎和肝外肿瘤组患者 (P <0 0 1) ,但与急性肝炎组相比未见差别 (P >0 0 5 ) ;Alb基因在Ⅰ、Ⅱ和Ⅲ期肝癌中阳性率分别为 33%、37%和77% ;在肝内有无转移组间差异明显 (P <0 0 5 ) ,在伴肝外转移肝癌中全数阳性 ;在AFP <5 0 μg/L肝癌中阳性率为 17% ;Alb基因检出率与肝癌大小间无明显差异 (P >0 0 5 )。结论 外周血肝细胞Alb基因分析为肝癌诊断、转移和监测术后复发的有用标志物。
Objective To investigate the value of albumin (Alb) gene in peripheral blood mononuclear cells in the diagnosis and metastasis of liver cancer. Methods The Alb gene fragment in hepatocellular carcinoma tissues and peripheral blood was amplified by reverse transcriptase nested polymerase chain reaction (PCR). The value of Alb gene in diagnosis and metastasis of hepatocellular carcinoma was analyzed. Results Twenty cases of hepatocellular carcinoma, pericarcinoma and distant cancer tissues showed significantly higher concentrations of total RNA compared with those in cancerous tissues (P <0.05). The detection rate of Alb gene was 100% in all liver tissues and 59.7% in peripheral blood of patients with liver cancer. Were significantly higher than those in cirrhosis, chronic hepatitis and extrahepatic tumor group (P <0.01), but no difference compared with acute hepatitis group (P> 0.05); Alb gene in stage I, II and III liver cancer The positive rates were 33%, 37% and 77% respectively. There was a significant difference between the groups with or without intrahepatic metastasis (P <0.05), all of them were positive in extrahepatic liver cancer with AFP <50 μg / L The positive rate of HCC was 17%. There was no significant difference between the detection rate of Alb gene and the size of HCC (P> 0.05). Conclusion Alb gene analysis of peripheral blood hepatocytes is a useful marker for the diagnosis, metastasis and monitoring of postoperative recurrence of HCC.