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目的:建立心源素胶囊的定性定量方法。方法:采用薄层色谱法对西洋参和五味子进行定性鉴别,用HPLC-ELSD测定心源素中有效成分三七皂苷R1和人参皂苷Rg1、Re、Rb1的含量;色谱条件:Discovery C18色谱柱,乙腈(10%水)-0.1%冰醋酸水溶液,梯度洗脱,流速1.0mL/min,柱温30℃;漂移管温度105.6℃,载气流速3.0L/min。结果:在薄层色谱鉴别中能准确鉴定出心源素中的五味子和西洋参;三七皂苷R1和人参皂苷Rg1、Re、Rb1的线性范围分别为715～35.75μg/mL、1500～75μg/mL、1514～75.7μg/mL、2928～146.4μg/mL,平均回收率分别为88.53%(RSD=2.68%)、100.61%(RSD=1.95%)、100.01%(RSD=2.03%)、100.28%(RSD=2.40%)。结论:该方法可作为心源素胶囊的质量控制方法。
Objective: To establish a qualitative and quantitative method of Xin Yuan Capsule. Methods: TLC and HPLC were used to identify the genus Panax quinquefolius and Schisandra chinense. The content of notoginsenoside R1 and ginsenoside Rg1, Re and Rb1 were determined by HPLC-ELSD. Chromatographic conditions were as follows: C18 column, acetonitrile (10% water) -0.1% aqueous glacial acetic acid gradient elution at a flow rate of 1.0 mL / min with a column temperature of 30 ° C .; a drift tube temperature of 105.6 ° C and a carrier gas flow rate of 3.0 L / min. Results: Schisandra sp. And Panax quinquefolium could be identified accurately by TLC. The linear ranges of notoginsenoside R1 and ginsenoside Rg1, Re and Rb1 were 715 ~ 35.75μg / mL and 1500 ~ 75μg / mL respectively , 1514 ~ 75.7μg / mL and 2928 ~ 146.4μg / mL respectively. The average recoveries were 88.53% RSD = 2.68%, 100.61% RSD = 1.95%, 100.01% RSD = 2.03% and 100.28% RSD = 2.40%). Conclusion: This method can be used as the quality control method of Xinyuan capsule.