超高灵敏显色反应及其应用的研究——食品中痕量锰的光度法测定

• 作 者: 陈秀华 朱慧贞 程小敏
• 机 构: 杭州大学化学系,杭州大学化学系,杭州大学化学系
• 出 处: 杭州大学学报(自然科学版)
• 发表日期: 1994年04期
• 关键词: 显色反应 比耳定律 CTMAB 食品中痕量 最大吸收峰 辅助剂 磺酸基 摩尔吸光系数 吸光值 离子缔合

本文研究了在表面活性剂CTMAB和辅助剂H_2O_2、phen存在下,T(4-MP)PS_4与Mn(Ⅱ)超高灵敏显色反应的光度法测定条件。在溶液pH 9.15—9.35,拂水溶中加热6_min,配合物稳定形成。试剂最大吸收峰和形成配合物后的试剂消失峰于420nm。表观摩尔吸光系数为2.23×10~7L·mol~(-1)cm~(-1)。Mn(Ⅱ)浓度在0—50ng/25ml范围内符合比耳定律。本法用于淀粉、茶汤中痕量锰的测定,结果满意。

In this paper, the spectrophotometric determination of ultra-sensitive color reaction of T (4-MP) PS_4 and Mn (Ⅱ) in the presence of surfactant CTMAB and H_2O_2, phen was studied. In the solution pH 9.15-9.35, fuming water solution heating 6_min, the complex formed stable. Reagent maximum absorption peak and the disappearance of the peak after the formation of the complex at 420nm. The apparent molar absorptivity is 2.23 × 10 ~ 7L · mol ~ (-1) cm ~ (-1). Beer’s law is obeyed for the concentration of Mn (Ⅱ) in the range of 0-50ng / 25ml. This method is used for the determination of trace manganese in starch and tea with satisfactory results.