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目的:建立诺替可滴耳液中诺氟沙星、替硝唑、氢化可的松的含量测定方法。方法:色谱柱:Kromasil C_(18)柱(250mm×4.6mm,5μm),流动相:甲醇-乙腈-0.025mol·L~(-1)磷酸溶液(用三乙胺调至pH至3.0)-注射用水,梯度洗脱。诺氟沙星,替硝唑,氢化可的松分别为288nm,318nm,245nm,流速:0.8ml·min~(-1),柱温:室温。结果:诺氟沙星、替硝唑、氢化可的松分别在30.0～300μg·ml~(-1)(r=0.999 3)、30.0～300μg·ml~(-1)(r=0.999 7)、5.0～50μg·ml~(-1)(r=0.999 5)浓度范围内,峰面积与浓度的线性关系良好;平均回收率分别为99.5%(RSD=0.61%),99.9%(RSD=0.80%)和99.4%(RSD=0.89%)。结论:所用方法简便快速、结果准确可靠,适用于该复方制剂中3组分的同时测定。
Objective: To establish a method for the determination of norfloxacin, tinidazole and hydrocortisone in nocodazole ear drops. Methods: Kromasil C_ (18) column (250 mm × 4.6 mm, 5 μm) was used as the mobile phase. The mobile phase consisted of methanol-acetonitrile-0.025 mol·L -1 phosphoric acid solution Water for injection, gradient elution. Norfloxacin, tinidazole and hydrocortisone were 288nm, 318nm and 245nm respectively, the flow rate was 0.8ml · min -1, and the column temperature was room temperature. Results: The concentrations of norfloxacin, tinidazole and hydrocortisone were in the range of 30.0-300μg · ml -1 (r = 0.999 3), 30.0-300μg · ml -1 (r = 0.999 7) (R = 0.999 5). The average recoveries were 99.5% (RSD = 0.61%) and 99.9% (RSD = 0.80 %) And 99.4% (RSD = 0.89%). Conclusion: The method is simple and rapid, accurate and reliable, suitable for simultaneous determination of three components in the compound preparation.