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目的了解新疆北疆地区哈萨克族人群Rh阴性血型表型血清学的多态性,为临床科学、安全输血提供参考依据。对新生儿溶血病也具有重要的临床意义。方法随机整群抽取新疆北疆地区不同部落哈萨克族人群的无关个体22 660人,采用试管法检测Rh D抗原。Rh D阴性样本,用间接抗球蛋白试验确认,用抗-C、抗-c、抗-E、抗-e单克隆抗血清检测表型。结果检出Rh(D)阴性个体673例,弱D21例,新疆北疆地区哈萨克族Rh(D)阴性率为2.97%,Rh阴性表型在ABO血型中的分布为O>B>A>AB,血清学表型频率dccee(85.74%)>d Ccee(10.55%)>dcc Ee(2.52%)>dc CEe(0.89%)>d CCee(0.30%)。单倍型基因频率dce(0.924 171)>d Ce(0.058 558)>dc E(0.013 614)>d CE(0.003 657)。结论新疆北疆地区哈萨克族人群Rh阴性血型表型分布,具有本民族特征。哈萨克族具有白种人和东方人渗透、融合的特征。
Objective To understand the serological polymorphism of Rh-negative blood group phenotype in Kazakh population in northern Xinjiang and to provide a reference for clinical science and safe blood transfusion. Hemolytic disease also has important clinical significance. Methods A total of 22 660 unrelated individuals from different tribes of Kazak ethnic group in northern Xinjiang were collected by random cluster analysis. The Rh D antigen was detected by in vitro test. Rh D-negative samples were confirmed by an indirect antiglobulin assay and phenotypes were detected with anti-C, anti-c, anti-E, anti-e monoclonal antiserum. Results The 673 Rh (D) negative individuals, 21 weak ones, and 2.97% Kazakh (Rh (D) negative) were detected in northern Xinjiang. The distributions of Rh negative phenotypes in ABO blood group were O> B> A> AB The serological phenotype frequency was dccee (85.74%)> d Ccee (10.55%)> dcc Ee (2.52%)> dc CEe (0.89%)> d CCee (0.30%). The haplotype frequency dce (0.924 171)> d Ce (0.058 558)> dc E (0.013 614)> d CE (0.003 657). Conclusion The distribution of Rh-negative blood type phenotype in Kazak nationality in northern Xinjiang is characterized by its ethnic characteristics. Kazakhs have the characteristics of infiltration and integration of white and oriental people.