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目的:探讨二苯乙烯苷抗动脉粥样硬化的可能机制。方法:载脂蛋白E基因敲除小鼠(apo E-/-小鼠)随机分为3组(n=6),模型对照组(高脂饮食),二苯乙烯苷小剂量组(高脂饮食+二苯乙烯苷20 mg/kg),二苯乙烯苷大剂量组组(高脂饮食+二苯乙烯苷40 mg/kg),人工饲养12周后行小鼠主动脉弓HE染色,确认模型对照组斑块形成。另外选用6只C57BL/6J小鼠作为正常对照组。根据分组,给予药物干预4周,给药期间全部换用普通饲料。实验结束前,取血清,并取主动脉。HE检测粥样斑块;生化法检测血脂;elisa法检测血清中oxLDL的浓度;western blot法检测、细胞间黏附分子-1(ICAM1-1)、白介素-1(IL-1)、肿瘤坏死因子-α(TNF-α)、凝集素样氧化型低密度脂蛋白受体(LOX-1)的表达。结果:(1)HE染色结果显示:空白对照组主动脉内膜未见斑块;模型对对照组斑块明显;二苯乙烯苷组斑块面积显著缩小。(2)模型对照组血脂上升,二苯乙烯苷组血脂有一定降低。(3)模型组血管组织ICAM1、IL-1、TNF-α、LOX-1表达上调,二苯乙烯苷组ICAM1、IL-1、TNF-α、LOX-1表达显著下调。结论:二苯乙烯苷能够通过降低血脂,抑制抑制ox-LDL与LOX-1,调整炎性介质的表达发挥其抗动脉粥样硬化的作用。
Objective: To investigate the possible mechanism of stilbene glycoside against atherosclerosis. Methods: Apolipoprotein E knockout mice (apo E - / - mice) were randomly divided into 3 groups (n = 6), model control group (high fat diet), low dose stilbene group Diet + stilbene glucoside 20 mg / kg), high dose of stilbene group (high-fat diet + stilbene glucoside 40 mg / kg), and then aortic arch was stained HE after artificial feeding for 12 weeks to confirm the model control group Block formation. In addition, 6 C57BL / 6J mice were selected as normal control group. According to the grouping, the patients were given drug intervention for 4 weeks. All the animals were switched to ordinary feed during the administration. Before the end of the experiment, serum was taken and the aorta was taken. Hematoxylin and eosin (HE) was used to detect the atherosclerotic plaque. Serum levels of oxLDL were determined by biochemical method. The concentration of oxLDL in serum was detected by elisa assay. The expressions of ICAM1-1, IL-1, -α (TNF-α), lectin-like oxidized low density lipoprotein receptor (LOX-1). Results: (1) The results of HE staining showed that there was no plaque in the aortic intima in the blank control group; the plaque in the control group was obvious; the plaque area in the stilbene glycoside group was significantly reduced. (2) The model control group increased blood lipids, stilbene glycosides group of blood lipids decreased. (3) The expression of ICAM1, IL-1, TNF-α and LOX-1 were upregulated in the model group. The expressions of ICAM1, IL-1, TNF-αand LOX-1 in stilbene glycoside group were significantly decreased. CONCLUSIONS: Stilbene glucoside can exert its anti-atherosclerosis effect by lowering blood lipids, inhibiting the inhibition of ox-LDL and LOX-1 and regulating the expression of inflammatory mediators.