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采用95%乙醇对刺桐中化学成分进行了分离提取,采用二氯甲烷、乙酸乙酯、正丁醇和水作为溶剂对95%乙醇提取物进行了进一步的分离提取,获得4个不同极性的提取部位。以抗坏血酸(VC)做为阳性对照,利用清除二苯代苦味酰基(DPPH)自由基法和铁离子还原/抗氧化能力(FRAP)法对4个不同极性的组分进行抗氧化活性评价,研究结果表明,中药刺桐具有较好的抗氧化能力,其中,乙酸乙酯部位(EVE)抗氧化能力最强,为此,我们利用硅胶柱色谱对乙酸乙酯部位(EVE)进行了进一步分离,共获得10个组分。采用上述活性评价的方法对所获得的乙酸乙酯10个组分进行了抗氧化活性评价,发现EVE-B组分抗氧化活性最高,其清除DPPH自由基能力(IC50=8.42μg·L-1)强于阳性对照VC(IC50=9.51μg·L-1),总抗氧化能力远远高于VC。
95% ethanol was used to separate and extract the chemical constituents from C. fortunei. 95% ethanol extracts were further separated and extracted with dichloromethane, ethyl acetate, n-butanol and water as solvent to obtain four different polarity Extract parts. Using ascorbic acid (VC) as a positive control, antioxidant activities of four fractions with different polarities were evaluated by DPPH free radical scavenging assay and ferric ion reduction / antioxidant activity (FRAP) The results showed that Eriantha cinnamomea has better anti-oxidative ability, of which ethyl acetate (EVE) has the strongest anti-oxidation ability. To this end, we further separated the ethyl acetate fraction (EVE) by silica gel column chromatography , A total of 10 components were obtained. The anti-oxidative activity of the 10 components of ethyl acetate obtained by the above activity evaluation method was evaluated. It was found that the antioxidant activity of EVE-B component was the highest, and its DPPH radical scavenging ability (IC50 = 8.42 μg · L -1 ) Was stronger than positive control VC (IC50 = 9.51μg · L-1), the total antioxidant capacity was much higher than that of VC.