论文部分内容阅读
1、米曲氨基酰化酶吸附于DEAE—萄聚糖凝胶制得固定化酶。2、考察了固定化酶的一些性质。固定化对氨基酰化酶的温度—活力关系没有影响;固定化酶的表观米氏常数与天然酶的米氏常数差别也不大;固定化酶的热稳定性提高5℃。3、固定化酶及天然酶水解乙酰—DL—色氨酸、苯丙氨酸、甲硫氨酸速度较快,缬氨酸、丙氨酸和α—氨基丁酸的衍生物居中,非天然氨基酸对甲氧苯乙氨酸的衍生物最慢。4、比较了固定化氨基酰化酶柱式法与分批法降解乙酰—DL—α—氨基丁酸的运转情况,表明酶柱在45℃,169小时运转过程中,能稳定地拆分底物。
1, amylose amino acylase adsorption DEAE-dextran gel immobilized enzyme. 2, examined some of the properties of immobilized enzyme. The immobilization had no effect on the temperature-activity relationship of aminoacylase. The difference between the apparent Michaelis constant of immobilized enzyme and that of natural enzyme was not significant. The thermostability of immobilized enzyme increased by 5 ℃. 3, immobilized enzyme and natural enzyme hydrolysis acetyl-DL-tryptophan, phenylalanine, methionine faster, valine, alanine and α-aminobutyric acid derivatives center, non-natural The amino acid p-methoxyphenetidine derivative is the slowest. Compared with immobilized aminoacylase column method and batch method, the operation of acetyl-DL-α-aminobutyric acid was degraded, indicating that the enzyme column can be stably disassembled at 45 ℃ and 169 hours Things.