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研究了云芝子实体氯仿提取物(CVFC)对人肝癌细胞株HepG2的体外抑制作用,并初步探讨了抑瘤机制。MTT结果显示,CVFC对HepG2细胞有明显的抑制作用,并呈明显的量效关系,半数抑制浓度为(75.83±3.96)μg.mL-1。荧光显微镜和透射电镜形态观察发现CVFC处理可使细胞发生细胞皱缩、核碎裂等凋亡特征;Annexin V/PI双染结果证实有胞膜磷脂酰丝氨酸的翻转,经150μg.mL-1 CVFC处理HepG2细胞24 h后早期凋亡率为17.82%,晚期凋亡率为22.59%,均显著高于对照组5.35%和3.75%。这些结果表明CVFC具有体外抑制HepG2细胞的活性,其抑瘤机制与诱导肿瘤细胞凋亡有关。
The in vitro inhibition of human hepatocellular carcinoma cell line HepG2 by chloroform extract (CVFC) of Polygonatum wallichii was studied, and the anti-tumor mechanism was preliminary discussed. The results of MTT showed that CVFC had a significant inhibitory effect on HepG2 cells with a significant dose-response relationship, with a median inhibitory concentration of (75.83 ± 3.96) μg.mL-1. Fluorescence microscopy and transmission electron microscopy morphological observation found that CVFC treatment cells can occur cell shrinkage, nuclear fragmentation and other apoptotic features; Annexin V / PI double staining confirmed the membrane phosphatidylserine turnover, the 150μg.mL-1 CVFC The early apoptosis rate of HepG2 cells was 17.82% after 24 h, and the late apoptosis rate was 22.59%, which were significantly higher than that of control group (5.35% and 3.75% respectively). These results indicate that CVFC can inhibit the activity of HepG2 cells in vitro, and its anti-tumor mechanism is related to the induction of apoptosis of tumor cells.