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目的观察化学指示卡与生物指示剂在压力蒸汽灭菌应用中的监测结果,选择压力蒸汽灭菌效果监测指示物。方法通过标准监测包和自制监测包方式,比较化学指示卡与生物指示剂监测压力蒸汽灭菌效果,结果在脉动真空式压力蒸汽灭菌器内常规灭菌参数条件下灭菌10 min,挑战包和自制包灭菌后的包外指示带均变色合格,爬行卡合格率均为100%,快速生物指示剂培养合格率均为100%。在132~134℃、206 k Pa、脉动2次、灭菌4 min临界参数下,挑战包爬行卡合格率为83.3%,挑战包的生物指示剂培养合格率63.3%。结论化学指示卡和生物指示剂监测压力蒸汽灭菌效果,在常规参数下无差别,但在临界灭菌参数下监测结果存在差异,提示生物指示剂监测更严格。
Objective To observe the monitoring results of the chemical indicator card and biological indicator in the application of pressure steam sterilization and select the monitoring indicator of the pressure steam sterilization effect. Methods By means of standard monitoring package and self-made monitoring package, the effect of pressure steam sterilization was monitored by comparing the chemical indicator card and biological indicator. The results were sterilized for 10 min under the conditions of conventional sterilization parameters in a pulsating vacuum pressure steam sterilizer. The challenge package And homemade package after sterilization outside the package with the indicator of discoloration pass, crawling card pass rate was 100%, rapid biological indicator training pass rate was 100%. Under the critical parameters of 132 ~ 134 ℃, 206 kPa, pulsation twice, and sterilization for 4 min, the passing rate of challenge crawls was 83.3%, and the challenge rate of biological indicator cultivation was 63.3%. Conclusion The chemical indicator card and biological indicator to monitor the effect of pressure steam sterilization, no difference under the conventional parameters, but under the critical sterilization parameters there are differences in monitoring results, suggesting that biological indicator monitoring more stringent.