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淋巴管内皮细胞(LECs)密切参与许多疾病的病理生理过程,如淋巴水肿、炎症、肿瘤淋巴结转移等。其体外分离培养技术将为探索相关疾病的细胞生物学和分子生物学提供重要的实验基础和丰富的细胞来源,从而为该疾病的预防和诊疗提供依据。近年来有关LECs体外分离培养的研究进展很快,且研究内容更加广泛和深入。本文就LECs体外分离培养的取材来源、分离培养方法及鉴定等多方面进行文献总结,为进一步研究和完善LECs体外分离培养提供参考。
Lymphatic endothelial cells (LECs) are closely involved in the pathophysiological processes of many diseases, such as lymphedema, inflammation, tumor lymph node metastases, and the like. Its in vitro isolation and culture technology will provide important experimental basis and abundant cell sources for exploring cell biology and molecular biology of related diseases and provide basis for the prevention and diagnosis of the disease. In recent years, the research on LECs isolation and culture in vitro progressed rapidly, and the research content is more extensive and in-depth. In this paper, LECs in vitro isolation and culture of the source, isolation and culture methods and identification and other aspects of literature review, in order to further study and improve LECs isolation and culture provide a reference.