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通过对天然多糖进行化学修饰,制备具有ATRP引发位点的多糖引发剂,然后通过原子转移自由基聚合,制备以天然多糖为骨架,以不同链长的阳离子聚合物为侧链的阳离子非病毒基因载体。对合成的材料进行细胞转染、细胞毒性测试,并对其它各种性能进行表征。各项表征的结果证明基于ATRP法合成的多糖基因载体能够很好地络合DNA,并且具有良好的pH缓冲能力以及生物相容性,与PEI和商业化转染试剂相比,转染效率更高,细胞毒性更低,在基因治疗中具有很好的应用前景。
A polysaccharide initiator having an ATRP-inducing site is prepared by chemically modifying a natural polysaccharide, and then a cationic non-viral gene having a natural polysaccharide as a skeleton and cationic polymers having different chain lengths as a side chain is prepared by atom transfer radical polymerization Vector. The synthesized material was transfected into cells, tested for cytotoxicity, and characterized for various other properties. The results of various characterizations demonstrated that the polysaccharide gene vector synthesized based on the ATRP method can well complex DNA, and has good pH buffering capacity and biocompatibility. Compared with PEI and commercial transfection reagent, transfection efficiency is more High, lower cytotoxicity, has a good prospect in gene therapy.