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目的探讨外源性谷胱甘肽(glutathione,GSH)对饮水砷暴露小鼠体内不同组织器官砷形态分布的影响。方法将实验小鼠随机分为对照组、单纯染砷组及低、中和高剂量GSH干预组。小鼠经饮水染砷4周,并于最后一周,在染砷同时腹腔注射不同剂量GSH。末次注射后24 h处死小鼠,取血、肝和脑组织。分别检测无机砷(iAs)、一甲基胂(MMA)和二甲基胂(DMA)的含量。结果GSH干预组小鼠的肝和血中iAs和MMA含量及血和脑中总砷含量(TAs)与单纯染砷组比较显著下降。GSH干预组小鼠的肝和血一甲基化率(PMR)和二甲基化率(SMR)明显升高。其中,高剂量GSH干预组小鼠的肝PMR和SMR及血SMR与单纯染砷组比较差异显著。结论外源性GSH可以促进肝中iAs的甲基化,加速砷化物从机体的排泄,从而可减少血中砷化物的含量,进而降低脑中砷化物的蓄积。
Objective To investigate the effects of exogenous glutathione (GSH) on the distribution of arsenic in different tissues and organs of arsenic-exposed mice. Methods The experimental mice were randomly divided into control group, arsenic group and low, medium and high dose GSH intervention groups. The mice were arsenic-stained with arsenic for 4 weeks, and in the last week, different doses of GSH were injected intraperitoneally while arsenic was stained. Twenty-four hours after the last injection, mice were killed and blood, liver and brain tissues were taken. The content of inorganic arsenic (iAs), monomethylarsine (MMA) and dimethyl arsine (DMA) were detected. Results The levels of iAs and MMA in liver and blood, and total arsenic (TAs) in blood and brain of GSH intervention group were significantly decreased compared with those of arsenic alone group. The liver and blood monomethylation rate (PMR) and dimethylation rate (SMR) in GSH intervention group were significantly increased. Among them, the high dose of GSH intervention group of mice liver PMR and SMR and blood SMR compared with simple staining arsenic group significant difference. Conclusion Exogenous GSH can promote the methylation of iAs in the liver and accelerate the excretion of arsenic compounds from the body, which can reduce the content of arsenic in blood and reduce the accumulation of arsenic in the brain.