Modification of Chiral Stationary Phases with L-Proline as a Selector for Ligand-exchange Chromatogr

• 出 处: Chemical Research in Chinese Universities
• 发表日期: 2009年06期
• 关键词: proline Proline silica Modification chiral stationary modifying allyl completed

Three chiral stationary phases(CSP1, CSP2 and CSP3)for ligand-exchange chromatography were prepared by firstly using dimethylchlorosilane as an endcapping reagent for decreasing residual silanol groups on the surface of silica gel, and then modifying the surface of silica gel with allyl glycidyl ether and alkenes through the hydrosilation reaction, and lastly introducing L-proline as a chiral selector. The enantiomer resolutions of 14 amino acids and 2 hydroxyl acids were completed on the CSPs by using an aqueous solution of Cu(Ac)2 as mobile phase at a flow rate of 1.0 mL/min and column temperature of 40 °C with detection at UV 254 nm. In terms of enantioselectivity α, column efficiency and resolution Rs, the chromatographic behaviors of the analytes on the CSPs were discussed via comparing them to those on the CSP4 prepared via the reference method. The results show that enantioselectivity α, column efficiency and resolution Rs of the analytes on the CSPs could be improved by using the above modifying method.

Three chiral stationary phases (CSP1, CSP2 and CSP3) for ligand-exchange chromatography were prepared by first using dimethylchlorosilane as an endcapping reagent for decreasing residual silanol groups on the surface of silica gel, and then modifying the surface of silica gel with allyl glycidyl ether and alkenes through the hydrosilation reaction, and lastly introducing L-proline as a chiral selector. The enantiomer resolutions of 14 amino acids and 2 hydroxyl acids were completed on the CSPs by using an aqueous solution of Cu (Ac) 2 as mobile phase at a flow rate of 1.0 mL / min and column temperature of 40 ° C with detection at UV 254 nm. In terms of enantioselectivity α, column efficiency and resolution Rs, the chromatographic behaviors of the analyzes on the CSPs were discussed via comparing them to those on the CSP4 prepared via the reference method. The results show that enantioselectivity α, column efficiency and resolution Rs of the analyzes on the CSPs could be improved by usin g the above modifying method.