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以亳州芍药鳞芽外植体为试材,对3个时间段(2月下旬至3月上旬、9月下旬至10月上旬、12月下旬至翌年1月上旬)的鳞芽进行了优化,并确定了亳州芍药组织培养的最佳取样时间;以2月下旬至3月上旬(第1阶段)鳞芽为试材,研究了5种鳞芽表面灭菌方法和IBA、NAA与BA组合的4因素2水平以及BA与KT组合的2因素2水平培养基组分筛选正交实验,以建立一套高效成熟的亳州芍药组织培养的快速繁殖技术体系。结果表明:2月下旬至3月上旬取样的亳州芍药鳞芽诱导成活率最高,采用0.13%HgCl2浸泡10min对亳州芍药萌发芽进行2次灭菌效果最好;亳州芍药鳞芽最佳的增殖出芽分化培养基为1/2MS+1.0mg·L-1 BA+0.5mg·L-1 KT+3.0%蔗糖+0.7%琼脂。
In order to optimize the scale buds of peony shoot explants in Bozhou for three time periods (late February to early March, late September to early October, late December to early January) And the optimal sampling time of tissue culture of Paeonia lactiflora in Bozhou was determined. Using the scale buds from late February to early March (stage 1) as test materials, five kinds of methods of surface sterilization of scale buds and combination of IBA, NAA and BA 4 factors and 2 levels and BA and KT combination of 2 factors and 2 levels of medium components selected orthogonal experiment to establish a set of efficient and mature Bozhou Paeoniflorin tissue culture rapid propagation technology system. The results showed that the highest survival rate was obtained in the sample from Bozhou peony blossoms buds from late February to early March. The best germination rate of Bozhou Paeonia lactiflora bud germinated twice after being soaked with 0.13% HgCl2 for 10 minutes was the best. The differentiation medium was 1 / 2MS + 1.0 mg · L -1 BA + 0.5 mg · L -1 KT + 3.0% sucrose + 0.7% agar.