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目的 了解异体脱钙骨基质颗粒、骨水泥复合牛骨形成蛋白质植入犬股骨微波诱导高温原位灭活骨缺损后的生物学过程 ,观察该复合材料在体内的变化。方法 微波诱导高温 5 0℃ ,2 0min ,原位灭活犬股骨中段 ,造成 2 .5cm× 1.0cm骨缺损 ,将复合材料植入骨缺损处 ,分别于术后 1,2 ,3,4和 6个月进行99Tcm 亚甲基二膦酸盐 (MDP)骨显像 ,并与X线、组织学检查比较。对侧单纯植入骨水泥作对照。结果 术后 1个月可见放射性浓集 ,2个月增多 ,3个月最多 ,持续到 4个月 ,6个月时减至 2个月水平。X线检查 :1个月时可见骨痂形成 ,3个月最多 ,6个月不再增加。组织学观察 :3个月时复合材料内有新生骨形成 ,脱钙骨颗粒部分被新生骨替代 ;至 6个月 ,复合材料内新生骨形成明显增多 ,脱钙骨颗粒大部分被新生骨替代。结论 复合材料植入体内能诱导新骨形成 ,最终与宿主骨形成“生物铆定”。99Tcm MDP骨显像是判断复合材料在体内生物学过程直观、可靠的方法
OBJECTIVE: To investigate the biological process of allogeneic decalcified bone matrix and bone cement-bound bovine bone formation protein in dogs induced by microwave in-situ inactivated bone defect at high temperature, and to observe the changes of the composite in vivo. Methods Microwave induced high temperature of 50 ℃, 20min, in situ inactivation of the middle part of the canine femur, resulting in 2.5cm × 1.0cm bone defects, the composite material implanted in the bone defect, respectively, after 1,2, 3,4 and 99Tcm methylene diphosphonate (MDP) bone imaging was performed at 6 months and compared with X-ray, histological examination. Opposite simple bone cement as a control. Results One month after the operation, radioactive concentration was observed, increasing in 2 months, up to 3 months, lasting to 4 months and decreasing to 2 months at 6 months. X-ray examination: callus visible at 1 month, up to 3 months, 6 months no longer increased. Histological observation: At 3 months, new bone was formed in the composite and the part of decalcified bone was replaced by new bone. At 6 months, the formation of new bone increased significantly in the composite, and most of the demineralized bone was replaced by new bone . Conclusion Composite implants can induce new bone formation in the body and finally form “biological riveting” with the host bone. 99Tcm MDP Bone Imaging is an Intuitive and Reliable Method to Determine the Biological Process of Composites in Vivo