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采用组织块培养的方法获得滑膜成纤维细胞；ＭＴＴ法检测不同滑膜细胞培养上清液对滑膜成纤维细胞增殖反应的影响。结果：佐剂性关节炎（ＡＡ）大鼠不同稀释度滑膜细胞培养上清液均可促进成纤维细胞增殖反应，其最适稀释度为１∶４０；ＴＢＬ组的滑膜细胞培养上清液对成纤维细胞增殖无明显影响；甲氨喋呤组滑膜细胞培养上清液对成纤维细胞增殖有轻度的促进作用；消炎痛组滑膜细胞培养上清液对成纤维细胞的增殖具有明显的促进作用。提示：ＡＡ大鼠关节骨质破坏可能与滑膜细胞分泌细胞因子和炎症介质，促进成纤维细胞增殖有关；ＴＢＬ治疗ＲＡ的机理之一可能是通过下调滑膜细胞的分泌功能，使ＡＡ大鼠滑膜成纤维细胞的过度增殖恢复正常。
Synovial fibroblasts were obtained by tissue block culture; MTT assay was used to examine the effects of different synovial cell culture supernatants on proliferation of synovial fibroblasts. RESULTS: Adjuvant arthritis (AA) rats’ synovial cell culture supernatants at different dilutions could promote the proliferation of fibroblasts with an optimal dilution of 1:40; synovial cell culture supernatant in TBL group. The liquid had no effect on the proliferation of fibroblasts; the culture supernatant of synoviocytes of methotrexate had a slight promotion effect on the proliferation of fibroblasts; the proliferation of fibroblasts on the supernatants of synoviocytes of indomethacin group. Has a clear role in promoting. It is suggested that the destruction of joint bone in AA rats may be related to synovial cells secreting cytokines and inflammatory mediators and promoting the proliferation of fibroblasts. One of the mechanisms of TBL in the treatment of RA may be through the down-regulation of the secretory function of synovial cells to make AA rats. Hyperplasia of synovial fibroblasts returned to normal.