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以黑龙江省29个大豆育种单位的103份已鉴定大豆灰斑病3个生理小种抗性的大豆品种(系)为材料,选择与大豆灰斑病抗病基因连锁的19个SSR标记检测,获得等位变异数86个,每个标记检测到的等位变异数分布在2~6个之间,平均为4.4个。应用遗传统计软件(Genetics Statistics 3.0)分析表明,标记的多样性指数介于0.198~0.751之间,平均多样性指数为0.606。品种(系)特异指数差异较大,介于46.592~481.541之间,平均为87.415。根据标记的等位基因数,使用ID Analysis1.0软件分析表明,利用与大豆抗灰斑病基因连锁的7个SSR标记(Satt565、Satt547、Satt431、Sct_186、SOYGPATR、Satt244、Sat_151)就能有效区分各品种(系),因此利用这7个标记构建了供试品种(系)的分子身份证。
A total of 19 SSR markers linked to the resistance genes of soybean gray spot were selected from 103 soybean cultivars (lines) with resistance to 3 soybean cultivars identified by soybean gray spot disease in 29 soybean breeding units in Heilongjiang Province. The number of alleles obtained was 86, and the number of alleles detected by each marker ranged from 2 to 6 with an average of 4.4. The results of Genetics Statistics 3.0 showed that the diversity index of markers ranged from 0.198 to 0.751, and the average diversity index was 0.606. Varieties (lines) specific index difference is large, ranging between 46.592 ~ 481.541, with an average of 87.415. Based on the number of alleles tagged, ID Analysis 1.0 software analysis showed that the seven SSR markers (Satt565, Satt547, Satt431, Sct_186, SOYGPATR, Satt244, Sat_151) All varieties (lines), so the use of these seven markers constructed test varieties (lines) of the molecular ID.