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目的探讨胎盘性激素结合球蛋白(sex hormone-binding globulin,SHBG)基因启动子(TAAAA)n重复多态与妊娠期糖尿病(gestational diabetes mellitus,GDM)的关系。方法选择2011年1月至2013年2月在中国医科大学附属盛京医院单胎分娩女婴GDM孕妇129例(GDM组)和健康孕妇119例(对照组),采用基因片段分析加测序法检测胎盘SHBG基因启动子(TAAAA)n重复多态长度和重复次数,两组间进行比较。结果两组孕妇胎盘均检测到(TAAAA)n重复次数为6、7、8、9、10次重复5个等位基因,6/6、6/7、6/8、6/9、6/10、7/7、7/8、7/9、7/10、8/8、8/9、8/10、9/9、9/10共14种基因型。两组间5种等位基因构成比及各等位基因频率差异均无统计学意义(P>0.05)。GDM组胎盘长链重复基因型频率虽然高于短链,但差异无统计学意义(χ~2=2.149,P=0.143)。结论胎盘SHBG基因启动子(TAAAA)n重复多态与妊娠期糖尿病无关。
Objective To investigate the relationship between TAAAA repeat polymorphism and gestational diabetes mellitus (GDM) in sex hormone-binding globulin (SHBG) gene. Methods From January 2011 to February 2013, 129 pregnant women (GDM group) and 119 healthy pregnant women (single female) with single-childbirth were enrolled in Shengjing Hospital, China Medical University from January 2011 to February 2013, Placental SHBG gene promoter (TAAAA) n repeat polymorphism length and number of repetitions, were compared between the two groups. Results There were 6, 7, 8, 9 and 10 repeats of 5 alleles in the placenta of both groups (TAAAA) n, 6/6, 6/7, 6/8, 6/9, 10, 7/7, 7/8, 7/9, 7/10, 8/8, 8/9, 8/10, 9/9 and 9/10. There were no significant differences in the proportions of the five alleles and the allele frequencies between the two groups (P> 0.05). The placenta long chain repeat genotype frequency in GDM group was higher than that in short chain, but the difference was not statistically significant (χ ~ 2 = 2.149, P = 0.143). Conclusion The placental SHBG gene promoter (TAAAA) n repeat polymorphism has nothing to do with gestational diabetes mellitus.